In vitro biocompatibility of a ferrimagnetic glass-ceramic for hyperthermia application

被引:30
作者
Bretcanu, Oana [1 ,7 ]
Miola, Marta [1 ]
Bianchi, Claudia L. [2 ]
Marangi, Ida [3 ,8 ]
Carbone, Roberta [3 ,9 ]
Corazzari, Ingrid [4 ,5 ]
Cannas, Mario [6 ]
Verne, Enrica [1 ]
机构
[1] Politecn Torino, Inst Mat Phys & Engn, Appl Sci & Technol Dept, Cso Duca Abruzzi 24, I-10129 Turin, Italy
[2] Univ Milan, Dept Chem, Via Golgi 19, I-20133 Milan, Italy
[3] European Inst Oncol, Dept Expt Oncol, Milan, Italy
[4] Univ Turin, Dept Chem, Via Pietro Giuria 7, I-10125 Turin, Italy
[5] G Scansetti Interdept Ctr Studies Asbesto & Tox P, Via Pietro Giuria 7, I-10125 Turin, Italy
[6] Univ Piemonte Orientale, Dept Med Sci Human Anat, Novara, Italy
[7] Newcastle Univ, Sch Mech & Syst Engn, Newcastle Upon Tyne, Tyne & Wear, England
[8] ATS INSUBRIA, Sede Terr Varese,Via O Rossi 9, I-21100 Varese, Italy
[9] Tethis SpA Genextra Grp, Via Russoli 3, I-20143 Milan, Italy
来源
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2017年 / 73卷
关键词
Ferrimagnetic; Glass-ceramic; Magnetite; Cell culture; Hyperthermia; Cancer; MAGNETIC-PROPERTIES; BIOACTIVE GLASSES; HEALTH-HAZARD; CANCER; DIFFERENTIATION; CELLS; IRON;
D O I
10.1016/j.msec.2016.12.105
中图分类号
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号
082905 [生物质能源与材料]; 100103 [病原生物学];
摘要
Ferrimagnetic glass-ceramics containing magnetite crystals were developed for hyperthermia applications of solid neoplastic tissue. The present work is focused on in vitro evaluation of the biocompatibility of these materials, before and after soaking in a simulated body fluid (SBF). X-ray diffraction, scanning electron microscopy, atomic absorption spectrophotometry, X-ray photoelectron spectrometry and pH measurements were employed in glass-ceramic characterisation. The free-radical mediated reactivity of the glass-ceramic was evaluated by Electron Paramagnetic Resonance (EPR) spin trapping. Cell adhesion and proliferation tests were carried out by using 313 murine fibroblasts. Cytotoxicity was performed by qualitative evaluation of human bone osteosarcoma cells U2OS cell line. The results show that almost two times more 313 cells proliferated on the samples pretreated in SBF, compared with the untreated specimens. Moreover a decrease of confluence was observed at 48 and 72 h for U2OS cells exposed to the untreated glass-ceramic, while the powder suspensions of glass-ceramic pre-treated in SBF did not influence the cell morphology up to 72 h of exposition. The untreated glass-ceramic exhibited Fenton-like reactivity, as well as reactivity towards formate molecule. After pre-treatment with SBF the reactivity towards formate was completely suppressed. The concentration of iron released into the SBF solution was below 0.1 ppm at 37 degrees C, during one month of soaking. The different in vitro behaviour of the samples before and after SBF treatment has been correlated to the bioactive glass-ceramic surface modifications as detected by morphological, structural and compositional analyses. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:778 / 787
页数:10
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