Effects of human antithymocyte globulin on acetylcholine synthesis, its release and choline acetyltransferase transcription in a human leukemic T-cell line

被引：28

作者：

Fujii, T ^{[1
]}

Ushiyama, N ^{[1
]}

Hosonuma, K ^{[1
]}

Suenaga, A ^{[1
]}

Kawashima, K ^{[1
]}

机构：

[1] Kyoritsu Coll Pharmaceut Sci, Dept Pharmacol, Tokyo 1058512, Japan

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2002年 / 128卷 / 1-2期

关键词：

acetylcholine; choline acetyltransferase; T-lymphocyte;

D O I：

10.1016/S0165-5728(02)00111-X

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Lymphocytes possess an independent, nonneuronal cholinergic system. In the present study, we investigated the short- and long-term effects of antithymocyte globulin (ATG)-Fresenius (ATG-F), a human antithymocyte globulin that binds to CD2, CD7 and CD11a, on acetylcholine (ACh) synthesis and transcription of choline acetyltransferase (ChAT) in CCRF-CEM cells, a human leukemic T-cell line. In the short-term (6 h), ATG-F enhanced ACh release, likely through transient increases in intracellular Ca2+ ([Ca2+](i)) mediated by CD7, which led to declines in intracellular ACh content. By 48 h, however, the ACh content had increased as compared to control due to up-regulation of ChAT expression mediated by CD11a. (C) 2002 Elsevier Science B.V All rights reserved.

引用

页码：1 / 8

页数：8

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