Induction of choline acetyltransferase mRNA in human mononuclear leukocytes stimulated by phytohemagglutinin, a T-cell activator

被引:87
作者
Fujii, T
Yamada, S
Watanabe, Y
Misawa, H
Tajima, S
Fujimoto, K
Kasahara, T
Kawashima, K
机构
[1] Kyoritsu Coll Pharmaceut Sci, Dept Pharmacol, Minato Ku, Tokyo 105, Japan
[2] Kyoritsu Coll Pharmaceut Sci, Dept Biochem, Minato Ku, Tokyo 105, Japan
[3] Tokyo Metropolitan Inst Neurosci, Dept Neurol, Tokyo 183, Japan
关键词
acetylcholine; choline acetyltransferase; interleukin-2; mononuclear leukocytes; radioimmunoassay; T-lymphocyte;
D O I
10.1016/S0165-5728(97)00195-1
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The induction of mRNA for choline acetyltransferase (ChAT), which catalyzes acetylcholine (ACh) synthesis was investigated in human mononuclear leukocytes (MNL) stimulated by phytohemagglutinin (PHA), a T-cell activator, using the reverse transcription-polymerase chain reaction. Stimulation of MNL by PHA induced the expression of ChAT mRNA, and potentiated ACh synthesis. ChAT mRNA induction required mon time than the induction of interleukin-2 mRNA. Expression of the gene encoding the vesicular ACh transporter, which mediates ACh transport in cholinergic neurons, was not observed in PHA-stimulated MNL, suggesting that the mechanisms controlling ACh release from T-lymphocytes differ from those in cholinergic neurons. These findings demonstrate that activation of T-lymphocytes up-regulates ACh synthesis in the blood, and suggest that ACh plays an important role as a neuroimmunomodulator besides its role as a neurotransmitter. (C) 1998 Elsevier Science B.V.
引用
收藏
页码:101 / 107
页数:7
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