Six putative two-component regulatory systems isolated from Lactococcus lactis subsp cremoris MG1363

被引：55

作者：

O'Connell-Motherway, M

van Sinderen, D ^{[1
]}

Morel-Deville, F

Fitzgerald, GF

Ehrlich, SD

Morel, P

机构：

[1] Natl Univ Ireland Univ Coll Cork, Dept Microbiol, Cork, Ireland

[2] Natl Univ Ireland Univ Coll Cork, Dept Food Sci & Technol, Cork, Ireland

[3] Natl Univ Ireland Univ Coll Cork, Natl Food Biotechnol Ctr, Cork, Ireland

[4] INRA, Lab Rech Viande, F-78352 Jouy En Josas, France

[5] INRA, Lab Genet Microbienne, F-78352 Jouy En Josas, France

来源：

MICROBIOLOGY-SGM | 2000年 / 146卷

关键词：

Lactococcus lactis; signal transduction; response regulator; two-component regulatory systems; histidine protein kinase;

D O I：

10.1099/00221287-146-4-935

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The genetic elements specifying six putative two-component regulatory systems (2CSs) were identified on the chromosome of Lactococcus lactis MG1363. These 2CSs appear to represent distinct loci, each containing a histidine kinase and response-regulator-encoding gene pair. Transcriptional analysis of the six 2CSs was performed either by generating transcriptional fusions to a reporter gene or by primer extension. Two of the systems appeared to be expressed constitutively at a high level, whilst the remaining four exhibited growth-phase-dependent expression. insertional mutagenesis established that the two constitutively expressed 2CSs are necessary for normal cell growth and/or survival. Mutational analysis of the remaining four systems revealed that they are implicated in susceptibility to extreme pH. osmotic or oxidative conditions, or the regulation of phosphatase activity in L. lactis.

引用

页码：935 / 947

页数：13

共 52 条

[1] PROTEIN HISTIDINE KINASES AND SIGNAL-TRANSDUCTION IN PROKARYOTES AND EUKARYOTES [J].