Thermally responsive poly (N-isopropylacrylamide) monolayer on gold:: synthesis, surface characterization, and protein interaction/adsorption studies

被引:54
作者
Cho, EC [1 ]
Kim, YD [1 ]
Cho, K [1 ]
机构
[1] Pohang Univ Sci & Technol, Polymer Res Inst, Dept Chem Engn, Pohang 790784, South Korea
关键词
poly(N-isopropylacrylamide); monolayer; protein adhesion;
D O I
10.1016/j.polymer.2004.02.052
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
This report describes a novel method for preparing a thermally responsive poly(N-isopropylacrylamide) (PNiPAM) monolayer on a gold surface, and demonstrates the function of this monolayer in aqueous media. Thiol (-SH) terminated PNiPAM was synthesized by UV polymerization followed by hydrolysis, and a monolayer of this polymer (2.84 +/- 0.2 nm) was prepared on a gold substrate by simply dipping a precleaned gold plate into an aqueous solution of the PNiPAM. Cyclic voltametry and atomic force microscopy studies showed that the gold surface was well covered by the PNiPAM chains, and X-ray photoelectron spectroscopic data showed that this monolayer was chemisorbed on the gold surface. Studies of the water contact angle, protein interaction, and protein adsorption on the PNiPAM monolayer demonstrated that this monolayer shows a temperature dependence of the interfacial properties in aqueous media. (C) 2004 Elsevier Ltd. All rights reserved.
引用
收藏
页码:3195 / 3204
页数:10
相关论文
共 54 条
[41]   MICROCALORIMETRIC DETECTION OF LOWER CRITICAL SOLUTION TEMPERATURES IN AQUEOUS POLYMER-SOLUTIONS [J].
SCHILD, HG ;
TIRRELL, DA .
JOURNAL OF PHYSICAL CHEMISTRY, 1990, 94 (10) :4352-4356
[42]   Direct surface force and contact angle measurements of an adsorbed polymer with a lower critical solution temperature [J].
Schmitt, FJ ;
Park, C ;
Simon, J ;
Ringsdorf, H ;
Israelachvili, J .
LANGMUIR, 1998, 14 (10) :2838-2845
[43]   Preparation of poly(N-isopropylacrylamide)-monolayer-protected gold clusters:: Synthesis methods, core size, and thickness of monolayer [J].
Shan, J ;
Nuopponen, M ;
Jiang, H ;
Kauppinen, E ;
Tenhu, H .
MACROMOLECULES, 2003, 36 (12) :4526-4533
[44]   Atomic force microscopy and X-ray reflectivity studies of albumin adsorbed onto self-assembled monolayers of hexadecyltrichlorosilane [J].
Sheller, NB ;
Petrash, S ;
Foster, MD ;
Tsukruk, VV .
LANGMUIR, 1998, 14 (16) :4535-4544
[45]  
SHIBAYAMA M, 1993, ADV POLYM SCI, V109, P1
[46]  
SOCRATES G, 1994, INFRARED CHARACTERIS, P69
[47]   Synthesis and characterization of injectable poly(N-isopropylacrylamide)-based hydrogels that support tissue formation in vitro [J].
Stile, RA ;
Burghardt, WR ;
Healy, KE .
MACROMOLECULES, 1999, 32 (22) :7370-7379
[48]   Effects of non-uniform solvation on thermal response in poly(N-isopropylacrylamide) gels [J].
Suetoh, Y ;
Shibayama, M .
POLYMER, 2000, 41 (02) :505-510
[49]   DYNAMIC CONTACT-ANGLE MEASUREMENT OF TEMPERATURE-RESPONSIVE SURFACE-PROPERTIES FOR POLY(N-ISOPROPYLACRYLAMIDE) GRAFTED SURFACES [J].
TAKEI, YG ;
AOKI, T ;
SANUI, K ;
OGATA, N ;
SAKURAI, Y ;
OKANO, T .
MACROMOLECULES, 1994, 27 (21) :6163-6166
[50]   Molecular dynamics study of polymer-water interaction in hydrogels .1. Hydrogen-bond structure [J].
Tamai, Y ;
Tanaka, H ;
Nakanishi, K .
MACROMOLECULES, 1996, 29 (21) :6750-6760