Unexpected substitution of dominant rotavirus G genotypes in French hospitalized children over five consecutive seasons

被引:20
作者
de Rougemont, A. [1 ,2 ]
Kaplon, J. [2 ]
Lebon, P. [3 ]
Huet, F. [4 ]
Denis, F. [5 ]
Alain, S. [5 ]
Fourcade, L. [6 ]
Grosjean, J. [6 ]
El-Hajje, M. -J. [7 ]
Gendrel, D. [7 ]
Pothier, P. [1 ,2 ]
机构
[1] Ctr Hosp Univ Dijon, Virol Lab, Ctr Natl Reference Virus Enter, F-21070 Dijon, France
[2] Univ Hosp, Virol Lab, Natl Reference Ctr Enter Viruses, F-21000 Dijon, France
[3] Hop St Vincent de Paul, Assistance Publ Hop Paris, Virol Lab, F-75014 Paris, France
[4] Univ Hosp, Dept Pediat, F-21000 Dijon, France
[5] Univ Hosp, Virol Lab, F-87000 Limoges, France
[6] Univ Hosp, Dept Pediat, F-87000 Limoges, France
[7] Hop St Vincent de Paul, Assistance Publ Hop Paris, Dept Pediat, F-75014 Paris, France
关键词
POLYMERASE-CHAIN-REACTION; MOLECULAR CHARACTERIZATION; 3-YEAR PERIOD; MAJOR CAUSE; STRAINS; VP7; DIVERSITY; DIARRHEA; GASTROENTERITIS; EPIDEMIOLOGY;
D O I
10.1007/s10096-008-0640-1
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The study was designed to evaluate the circulation of group A rotaviruses in French hospitalized children, and to detect unusual strains. This prospective study was conducted from 2001 to 2006 in children consulting for acute diarrhea at the pediatric emergency department in three French University Hospitals. The rotaviruses were detected by rapid test and genotyped by RT-PCR on the basis of their outer capsid proteins VP4 (P-type) and VP7 (G-type). The stools from 757 children were analyzed. G1P[8] strains were predominant (44.0%), followed by G9P[8] (17.7%), G3P[8] 13.1%, G4P[8] (9.5%), and G2P[4] (1.8%); mixed rotavirus infections occurred in 2.3%. G9 rotaviruses emerged during the 2004-2005 season (73.4%) and remained the second most prevalent strains. Few unusual strains, G6, G8, G12 and P[6]-types, were detected. The monitoring of rotavirus infections should be maintained to document strain distribution and to assess the emergence of new reassortants that may not respond to current rotavirus vaccines.
引用
收藏
页码:403 / 407
页数:5
相关论文
共 36 条
  • [1] Molecular characterization of the genotype G9 human rotavirus strains recovered in Palermo, Italy, during the winter of 1999-2000
    Arista, S
    Giammanco, GM
    De Grazia, S
    Migliore, MC
    Martella, V
    Cascio, A
    [J]. EPIDEMIOLOGY AND INFECTION, 2004, 132 (02) : 343 - 349
  • [2] Heterogeneity and temporal dynamics of evolution of G1 human rotaviruses in a settled population
    Arista, Serenella
    Giammanco, Giovanni M.
    De Grazia, Simona
    Ramirez, Stefania
    Lo Biundo, Concetta
    Colomba, Claudia
    Cascio, Antonio
    Martella, Vito
    [J]. JOURNAL OF VIROLOGY, 2006, 80 (21) : 10724 - 10733
  • [3] Dominating prevalence of P[8],G1 and P[8],G9 rotavirus strains among children admitted to hospital between 2000 and 2003 in Budapest, Hungary
    Bányai, K
    Gentsch, JR
    Schipp, R
    Jakab, F
    Meleg, E
    Mihály, I
    Szücs, G
    [J]. JOURNAL OF MEDICAL VIROLOGY, 2005, 76 (03) : 414 - 423
  • [4] Bishop R.F., 1994, Viral Infections of the Gastrointestinal Tract, P131
  • [5] G and P genotyping of rotavirus strains circulating in France over a three-year period: Detection of G9 and P[6] strains at low frequencies
    Bon, F
    Fromantin, C
    Aho, S
    Pothier, P
    Kohli, E
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (04) : 1681 - 1683
  • [6] VP7 and VP4 genotypes among rotavirus strains recovered from children with gastroenteritis over a 3-year period in Valencia, Spain
    Buesa, J
    de Souza, CO
    Asensi, M
    Martínez, C
    Prat, J
    Gil, MT
    [J]. EUROPEAN JOURNAL OF EPIDEMIOLOGY, 2000, 16 (06) : 501 - 506
  • [7] Virus diversity in a winter epidemic of acute diarrhea in France
    Chikhi-Brachet, R
    Bon, F
    Toubiana, L
    Pothier, P
    Nicolas, JC
    Flahault, A
    Kohli, E
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (11) : 4266 - 4272
  • [8] Molecular and serologic characterization of novel serotype G8 human rotavirus strains detected in Blantyre, Malawi
    Cunliffe, NA
    Gentsch, JR
    Kirkwood, CD
    Gondwe, JS
    Dove, W
    Nakagomi, O
    Nakagomi, T
    Hoshino, Y
    Bresee, JS
    Glass, RI
    Molyneux, ME
    Hart, CA
    [J]. VIROLOGY, 2000, 274 (02) : 309 - 320
  • [9] Sensitivity and specificity of the VIKIA® Rota-Adeno immuno-chromatographic test (bioMerieux) and the ELISA IDEIA™ Rotavirus kit (Dako) compared to genotyping
    de Rougemont, A.
    Kaplon, J.
    Billaud, G.
    Lina, B.
    Pinchinat, S.
    Derrough, T.
    Caulin, E.
    Pothier, P.
    Floret, D.
    [J]. PATHOLOGIE BIOLOGIE, 2009, 57 (01): : 86 - 89
  • [10] Genetic and antigenic variation of capsid protein VP7 of serotype G1 human rotavirus isolates
    Diwakarla, CS
    Palombo, EA
    [J]. JOURNAL OF GENERAL VIROLOGY, 1999, 80 : 341 - 344