Sensitivity and specificity of the VIKIA® Rota-Adeno immuno-chromatographic test (bioMerieux) and the ELISA IDEIA™ Rotavirus kit (Dako) compared to genotyping

被引：23

作者：

de Rougemont, A. ^{[1
]}

Kaplon, J. ^{[1
]}

Billaud, G. ^{[2
]}

Lina, B. ^{[2
]}

Pinchinat, S. ^{[3
]}

Derrough, T. ^{[4
]}

Caulin, E. ^{[4
]}

Pothier, P. ^{[1
]}

Floret, D. ^{[5
]}

机构：

[1] CHU Dijon, Virol Lab, Ctr Natl Reference Virus Enter, F-21070 Dijon, France

[2] Hosp Civils Lyon, Virol Lab, Ctr Biol Est, F-69500 Bron, France

[3] Biostatem, F-34160 Castries, France

[4] Sanofi Pasteur MSD, F-69007 Lyon, France

[5] Hosp Civils Lyon, Hop Edouard Herriot, Serv Urgence & Reanimat Pediat, F-69003 Lyon, France

来源：

PATHOLOGIE BIOLOGIE | 2009年 / 57卷 / 01期

关键词：

Virus; Rotavirus; Gastroentery; Diagnosis; Immuno-chromatographic test; ELISA test; Genotyping; POLYMERASE-CHAIN-REACTION; RAPID DETECTION; DISEASE; INFECTION; DIVERSITY; STRAINS; GASTROENTERITIS; DIARRHEA; ENGLAND; BURDEN;

D O I：

10.1016/j.patbio.2008.08.004

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

The performances of two diagnostic tests for rotavirus infection in stool samples were evaluated during a prospective study in children of less than 36 months in child-care centers of Lyon from November 2004 to May 2005. The VIKIA (R) Rota-Adeno immuno-chromatographic test (bioMerieux) and the ELISA IDEIA (TM) Rotavirus kit (Dako) were compared with a referral method, the genotyping. Fifty-seven stool samples were collected and analyzed by RT-PCR. The virus genome was detected in 29 samples. The most frequent genotypic combinations were G9P[8] with a prevalence of 75.9%. Sensitivity and specificity of the VIKIA (R) Rota-Adeno test and the ELISA IDEIA (TM) Rotavirus kit were strictly comparable and very good: 96.6% (83.0; 99.9) and 96.4% (81.6; 99.9), respectively. The immuno-chromatographic technique were in concordance with the ELISA tests in 93.6% of cases. Thus, the VIKIA(R) Rota-Adeno test is a good alternative for the occasional analysis of stool samples in ambulatory practice. (c) 2008 Elsevier Masson SAS.

引用

页码：86 / 89

页数：4

共 27 条

[1] Evaluation of seven immunochromatographic assays for the rapid detection of human rotaviruses in fecal specimens [J].

Bon, F. ;

Kaplon, J. ;

Metzger, M.-H. ;

Pothier, P. .

PATHOLOGIE BIOLOGIE, 2007, 55 (3-4) :149-153

[2] Unexpected substitution of dominant rotavirus G genotypes in French hospitalized children over five consecutive seasons [J].

de Rougemont, A. ;

Kaplon, J. ;

Lebon, P. ;

Huet, F. ;

Denis, F. ;

Alain, S. ;

Fourcade, L. ;

Grosjean, J. ;

El-Hajje, M. -J. ;

Gendrel, D. ;

Pothier, P. .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2009, 28 (04) :403-407

[3] Etiology of gastroenteritis in sentinel general practices in the Netherlands [J].

de Wit, MAS ;

Koopmans, MPG ;

Kortbeek, LM ;

van Leeuwen, NJ ;

Vinjé, J ;

van Duynhoven, YTHP .

CLINICAL INFECTIOUS DISEASES, 2001, 33 (03) :280-288

[4]

Estes M.K., 2007, Fields Virology, V2, P1917

[5] Epidemiology and burden of rotavirus diarrhea in day care centers in Lyon, France [J].

Fau, C. ;

Billaud, G. ;

Pinchinat, S. ;

Lina, B. ;

Kaplon, J. ;

Pothier, P. ;

Derrough, T. ;

Marcelon, L. ;

Largeron, N. ;

Caulin, E. ;

Bellemin, B. ;

Nong, T. Cao ;

Gaspard, C. ;

Mamoux, V. ;

Floret, D. .

ARCHIVES DE PEDIATRIE, 2008, 15 (07) :1183-1192

[6] Longitudinal study of rotavirus infection in child-care centres [J].

Ferson, MJ ;

Stringfellow, S ;

McPhie, K ;

McIver, CJ ;

Simos, A .

JOURNAL OF PAEDIATRICS AND CHILD HEALTH, 1997, 33 (02) :157-160

[7] Serotype diversity and reassortment between human and animal rotavirus strains:: Implications for rotavirus vaccine programs [J].

Gentsch, JR ;

Laird, AR ;

Bielfelt, B ;

Griffin, DD ;

Bányai, K ;

Ramachandran, M ;

Jain, V ;

Cunliffe, NA ;

Nakagomi, O ;

Kirkwood, CD ;

Fischer, TK ;

Parashar, UD ;

Bresee, JS ;

Jiang, B ;

Glass, RI .

JOURNAL OF INFECTIOUS DISEASES, 2005, 192 :S146-S159

[8] IDENTIFICATION OF GROUP-A ROTAVIRUS GENE-4 TYPES BY POLYMERASE CHAIN-REACTION [J].

GENTSCH, JR ;

GLASS, RI ;

WOODS, P ;

GOUVEA, V ;

GORZIGLIA, M ;

FLORES, J ;

DAS, BK ;

BHAN, MK .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (06) :1365-1373

[9] Introduction: Rotavirus from basic research to a vaccine [J].

Glass, RI ;

Lang, DR ;

Ivanoff, BN ;

Compans, RW .

JOURNAL OF INFECTIOUS DISEASES, 1996, 174 :S1-S2

[10] Amino acid substitution within the VP7 protein of G2 rotavirus strains associated with failure to serotype [J].

Gómara, MI ;

Cubitt, D ;

Desselberger, U ;

Gray, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (10) :3796-3798

← 1 2 3 →