Mlp2p, a component of nuclear pore attached intranuclear filaments, associates with Nic96p

被引：72

作者：

Kosova, B ^{[1
]}

Panté, N ^{[1
]}

Rollenhagen, C ^{[1
]}

Podtelejnikov, A ^{[1
]}

Mann, M ^{[1
]}

Aebi, U ^{[1
]}

Hurt, E ^{[1
]}

机构：

[1] Biochem Zentrum Heidelberg, D-69120 Heidelberg, Germany

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2000年 / 275卷 / 01期

关键词：

D O I：

10.1074/jbc.275.1.343

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A fraction of the yeast nucleoporin Nic96p is localized at the terminal ring of the nuclear basket. When Nic96p was affinity purified from glutaraldehyde-treated spheroplasts, it was found to be associated with Mlp2p. Mlp2p, together with Mlp1p, are the yeast Tpr homologues, which form the nuclear pore attached intranuclear filaments (Strambio-de-Castillia, C., Blobel, G., and Rout, M. P. (1499) J. Cell Biol. 144, 839-855). Double disruption mutants of MLP1 and MLP2 are viable and apparently not impaired in nucleocytoplasmic transport. However, overproduction of MLP1 causes nuclear accumulation of poly(A)(+) RNA in a chromatin-free area of the nucleus.

引用

页码：343 / 350

页数：8

共 67 条

[61] A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores [J].

Siniossoglou, S ;

Wimmer, C ;

Rieger, M ;

Doye, V ;

Tekotte, H ;

Weise, C ;

Emig, S ;

Segref, A ;

Hurt, EC .

CELL, 1996, 84 (02) :265-275

[62] Proteins connecting the nuclear pore complex with the nuclear interior [J].

Strambio-de-Castillia, C ;

Blobel, G ;

Rout, MP .

JOURNAL OF CELL BIOLOGY, 1999, 144 (05) :839-855

[63]

WILKEN N, 1995, EUR J CELL BIOL, V68, P211

[64] NUP358, A CYTOPLASMICALLY EXPOSED NUCLEOPORIN WITH PEPTIDE REPEATS, RAN-GTP BINDING-SITES, ZINC FINGERS, A CYCLOPHILIN-A HOMOLOGOUS DOMAIN, AND A LEUCINE-RICH REGION [J].

WU, J ;

MATUNIS, MJ ;

KRAEMER, D ;

BLOBEL, G ;

COUTAVAS, E .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (23) :14209-14213

[65] Three-dimensional architecture of the isolated yeast nuclear pore complex: Functional and evolutionary implications [J].

Yang, Q ;

Rout, MP ;

Akey, CW .

MOLECULAR CELL, 1998, 1 (02) :223-234

[66] A GIANT NUCLEOPORE PROTEIN THAT BINDS RAN/TC4 [J].

YOKOYAMA, N ;

HAYASHI, N ;

SEKI, T ;

PANTE, N ;

OHBA, T ;

NISHII, K ;

KUMA, K ;

HAYASHIDA, T ;

MIYATA, T ;

AEBI, U ;

FUKUI, M ;

NISHIMOTO, T .

NATURE, 1995, 376 (6536) :184-188

[67]

Zimowska G, 1997, J CELL SCI, V110, P927

← 1 2 3 4 5 6 7 →