DNA sequence analysis with droplet-based microfluidics

被引：99

作者：

Abate, Adam R. ^{[1
]}

Hung, Tony ^{[2
]}

Sperling, Ralph A. ^{[2
,3
]}

Mary, Pascaline ^{[2
]}

Rotem, Assaf ^{[2
,4
]}

Agresti, Jeremy J. ^{[2
]}

Weiner, Michael A. ^{[5
]}

Weitz, David A. ^{[2
,6
]}

机构：

[1] Univ Calif San Francisco, San Francisco, CA 94143 USA

[2] Harvard Univ, SEAS, Cambridge, MA 02138 USA

[3] Inst Mikrotech Mainz GmbH, D-55129 Mainz, Germany

[4] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA

[5] Affomix, Branford, CT USA

[6] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA

来源：

LAB ON A CHIP | 2013年 / 13卷 / 24期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

TECHNOLOGY; SYSTEMS; PHYSICS;

D O I：

10.1039/c3lc50905b

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Droplet-based microfluidic techniques can form and process micrometer scale droplets at thousands per second. Each droplet can house an individual biochemical reaction, allowing millions of reactions to be performed in minutes with small amounts of total reagent. This versatile approach has been used for engineering enzymes, quantifying concentrations of DNA in solution, and screening protein crystallization conditions. Here, we use it to read the sequences of DNA molecules with a FRET-based assay. Using probes of different sequences, we interrogate a target DNA molecule for polymorphisms. With a larger probe set, additional polymorphisms can be interrogated as well as targets of arbitrary sequence.

引用

页码：4864 / 4869

页数：6

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