General method for the synthesis of caged phosphopeptides:: Tools for the exploration of signal transduction pathways

被引：34

作者：

Rothman, DM ^{[1
]}

Vázquez, EM ^{[1
]}

Vogel, EM ^{[1
]}

Imperiali, B ^{[1
]}

机构：

[1] MIT, Dept Chem, Cambridge, MA 02139 USA

来源：

ORGANIC LETTERS | 2002年 / 4卷 / 17期

关键词：

D O I：

10.1021/ol0262587

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

[GRAPHIC] An interassembly approach for the synthesis of peptides containing 1-(2-nitrophenyl)ethyl-caged phosphoserine, -threonine, and -tyrosine has been developed. Photochemical uncaging of these peptides releases the 2-nitrophenylethyl protecting group to afford the corresponding phosphopeptide. The peptides described herein are based on phosphorylation sites of kinases involved in cell movement or cell cycle regulation and demonstrate the versatility of the method and compatibility with the synthesis of polypeptides, including a variety of encoded amino acids.

引用

页码：2865 / 2868

页数：4

共 24 条

[1] Kinetic and catalytic mechanisms of protein kinases [J].