Organic metabolites in exhaled human breath-A multivariate approach for identification of biomarkers in lung disorders

被引：78

作者：

Gaspar, Elvira M. ^{[1
]}

Lucena, Ana F. ^{[1
]}

da Costa, J. Duro ^{[2
]}

das Neves, H. Chaves ^{[3
]}

机构：

[1] Univ Nova Lisboa, Fac Sci & Technol, CQFB Requimte, Dept Chem, P-2825114 Monte De Caparica, Portugal

[2] Inst Portugues Oncol Francisco Gentil, Dept Pulm Dis, P-1070 Lisbon, Portugal

[3] Univ Nova Lisboa, Fac Sci & Technol, UBIA, P-2825114 Monte De Caparica, Portugal

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 14期

关键词：

Human breath; Exhaled air; Cancer biomarkers; Metabolite profiles; Lung cancer profiles; Hydrocarbons; SPME-GC-(TOF)-MS; Multivariate analysis; Pattern recognition; SOLID-PHASE MICROEXTRACTION; ON-FIBER DERIVATIZATION; QUANTITATIVE-ANALYSIS; HELICOBACTER-PYLORI; MASS-SPECTROMETRY; HUMAN BLOOD; CANCER; ARRAY;

D O I：

10.1016/j.chroma.2008.10.125

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The gas chromatographic profiles of exhaled air from lung cancer patients have been investigated. The breath from healthy volunteers, smokers and non-smokers, and lung cancer patients without treatment and under radio and/or chemotherapy, was collected using Tedlar bags. Different profiles for healthy people and cancer patients could be recognized by multivariate analysis and significant diagnostic compounds could be established. Target compounds showed to be linear and branched hydrocarbons between C-14 and C-24. Solid phase microextraction (SPME) coupled to gas chromatography mass spectrometry CC-(TOF)-MS was used. The method showed good precision (RSD below 26%) and limit of detection ranged from 0.04 to 8.0 ppb. These findings show a high potential for establishment of laboratorial screening methods. Validation studies in a larger number of patients are being done. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：2749 / 2756

页数：8

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