Protein-ligand mediated aggregation of nanoparticles: A study of synthesis and assembly mechanism

被引:39
作者
Costanzo, PJ
Patten, TE
Seery, TAP
机构
[1] Univ Calif Davis, Dept Chem, Davis, CA 95616 USA
[2] Univ Connecticut, Inst Mat Sci, Storrs, CT 06269 USA
关键词
D O I
10.1021/cm049811i
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Two difunctional, asymmetric PEG linkers were synthesized. and utilized to prepare watersoluble, biotinylated CdS@SiO2 particles with various surface contents of the biotin groups. Aqueous dispersions of these particles were prepared, and upon addition of avidin, aggregation was observed using DLS, TEM, and SEM analysis. The resulting aggregates were dense but also had fractal shapes. It was shown that single-angle DLS measurements at 90degrees were sufficiently accurate for quickly measuring the size of the aggregates as it evolved with time. Initial aggregation rates were measured as a function of surface biotin content of the nanoparticles and initial avidin concentration.. The initial rates displayed bell-shaped curve responses to variations in the avidin-to-particle ratio and biotin-to-avidin ratio, the shapes of which were attributed to the stoichiometric balance/imbalance of the avidin-to-biotin ratio in the system and to intraparticle and interparticle bridges that could sterically block avidin from reaching some biotin sites. The growth mechanism Was postulated to be a step-growth process.
引用
收藏
页码:1775 / 1785
页数:11
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