The division during bacterial sporulation is symmetrically located in Sporosarcina ureae

被引:14
作者
Zhang, L [1 ]
Higgins, ML [1 ]
Piggot, PJ [1 ]
机构
[1] TEMPLE UNIV,SCH MED,DEPT MICROBIOL & IMMUNOL,PHILADELPHIA,PA 19140
关键词
D O I
10.1046/j.1365-2958.1997.5341892.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Immunofluorescence microscopy was used to visualize the FtsZ band that marks the site of septation in Sporosarcina ureae. Image analysis indicated that the vegetative division was symmetrically located with respect to the ends of the cells. Fusions of lacZ to the sporulation loci, spollA and cotE, of Bacillus subtilis were introduced into S. ureae by mobilization of plasmids containing the fusions from Escherichia coli. The fusions showed similar patterns of sporulation-associated expression in S. ureae to those observed in B. subtilis. Formation of P-galactosidase encoded by the spollA-lacZ fusion made it possible to identify early sporulating cells by immunofluorescence microscopy. Analysis of the position of FtsZ bands in cells expressing spollA-lacZ indicated that the location of sporulation division was symmetrical with respect to the ends of the cells, in sharp contrast to the asymmetrical location of septation in sporulating Bacilli. it is inferred that asymmetry of location of the sporulation division is not essential for the compartmentalization of gene expression that follows the division.
引用
收藏
页码:1091 / 1098
页数:8
相关论文
共 35 条
[1]   FtsZ ring formation in fts mutants [J].
Addinall, SG ;
Bi, EF ;
Lutkenhaus, J .
JOURNAL OF BACTERIOLOGY, 1996, 178 (13) :3877-3884
[2]   FTSZ IN BACILLUS-SUBTILIS IS REQUIRED FOR VEGETATIVE SEPTATION AND FOR ASYMMETRIC SEPTATION DURING SPORULATION [J].
BEALL, B ;
LUTKENHAUS, J .
GENES & DEVELOPMENT, 1991, 5 (03) :447-455
[3]   FTSZ RING STRUCTURE ASSOCIATED WITH DIVISION IN ESCHERICHIA-COLI [J].
BI, E ;
LUTKENHAUS, J .
NATURE, 1991, 354 (6349) :161-164
[4]   ANALYSIS BY FLUORESCENCE MICROSCOPY OF THE DEVELOPMENT OF COMPARTMENT-SPECIFIC GENE-EXPRESSION DURING SPORULATION OF BACILLUS-SUBTILIS [J].
BYLUND, JE ;
ZHANG, L ;
HAINES, MA ;
HIGGINS, ML ;
PIGGOT, PJ .
JOURNAL OF BACTERIOLOGY, 1994, 176 (10) :2898-2905
[5]  
CORTON JC, 1987, J BACTERIOL, V169, P1
[6]   GENETIC SUPPRESSION ANALYSIS OF SIGMA(E)-INTERACTION WITH 3 PROMOTERS IN SPORULATING BACILLUS-SUBTILIS [J].
DIEDERICH, B ;
TATTI, KM ;
JONES, CH ;
BEALL, B ;
MORAN, CP .
GENE, 1992, 121 (01) :63-69
[7]  
ERRINGTON J, 1993, MICROBIOL REV, V57, P1
[8]   PHYLOGENETIC INTERRELATIONSHIPS OF ROUND-SPORE-FORMING BACILLI CONTAINING CELL-WALLS BASED ON LYSINE AND THE NON-SPORE-FORMING GENERA CARYOPHANON, EXIGUOBACTERIUM, KURTHIA, AND PLANOCOCCUS [J].
FARROW, JAE ;
WALLBANKS, S ;
COLLINS, MD .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1994, 44 (01) :74-82
[9]  
Fitz-James P.C., 1969, BACTERIAL SPORE, P39
[10]  
GHOLAMHOSEINIAN A, 1989, J BACTERIOL, V171, P5745