Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines

被引:43
作者
Camps, Jordi
Salaverria, Itziar
Garcia, Maria J.
Prat, Esther
Bea, Silvia
Pole, Jessica C.
Hernandez, Lluis
Del Rey, Javier
Cigudosa, Juan Cruz
Bernues, Marta
Caldas, Carlos
Colomer, Dolors
Miro, Rosa
Campo, Elias
机构
[1] Univ Barcelona, Dept Anat Patol, IDIBAPS, Seccio Hematopatol Hosp Clin, E-08036 Barcelona, Spain
[2] Univ Autonoma Barcelona, Dept Biol Cellular Fisiol & Immunol, Bellaterra 08193, Spain
[3] Univ Autonoma Barcelona, Inst Biotecnol & Biomed, Bellaterra 08193, Spain
[4] Univ Cambridge, Dept Oncol, Canc Genom Program, Hutchison MRC Res Ctr, Cambridge CB2 2XZ, England
[5] Univ Cambridge, Dept Pathol, Canc Genom Program, Hutchison MRC Res Ctr, Cambridge CB2 2XZ, England
[6] CNIO, Cytogenet Unit, Madrid 28029, Spain
[7] Hosp Univ Son Dureta, Servei Genet, Palma de Mallorca 07014, Spain
基金
英国生物技术与生命科学研究理事会;
关键词
mantle-cell lymphoma; chromosomal instability; cell lines; comparative genomic hybridization; multicolor-FISH; cell cross-contamination; cell fusion;
D O I
10.1016/j.leukres.2005.11.013
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Mantle-cell lymphoma (MCL) is genetically characterized by 11q13 chromosomal translocations involving the CCND1 gene. We have characterized five MCL cell lines, JVM-2, GRANTA-519, REC-1, JEKO-1, and NCEB-1, combining metaphase and array comparative genomic hybridization, multicolor-FISH, and molecular analysis. Our results revealed common gained regions at 2p14, 9q31.2-qter, 11q13.1-q21, 13q14-q21.2, 13q34-qter and 18q21.1-q22.1, and losses at 1p21.2-p31.1, 2p11.2, 8p21.2-pter, 9p21.3-pter, 11q23.3-qter, 17p11.2-pter, and 17q21.2-q22.2. All cell lines except JVM-2, displayed moderate or high numerical chromosome instability. In addition, an ongoing level of chromosome rearrangements was observed in REC-1. Surprisingly, NCEB-1 carried several stable mouse chromosomes and showed expression of both human and murine bcl-2 protein. Our findings indicate that these cell lines represent three patterns of chromosome evolution in MCL and may be useful to understand the pathogenesis of this neoplasm. (c) 2005 Elsevier Ltd. All rights reserved.
引用
收藏
页码:923 / 934
页数:12
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