Fluorescence Quenching by Photoinduced Electron Transfer: A Reporter for Conformational Dynamics of Macromolecules

被引：438

作者：

Doose, Soren ^{[1
]}

Neuweiler, Hannes ^{[2
]}

Sauer, Markus ^{[1
]}

机构：

[1] Univ Bielefeld, D-33619 Bielefeld, Germany

[2] MRC Ctr Prot Engn, Cambridge CB2 0QH, England

来源：

CHEMPHYSCHEM | 2009年 / 10卷 / 9-10期

关键词：

biopolymer conformational dynamics; fluorescence spectroscopy; molecular diagnostics; photoinduced electron transfer; protein folding; UNFOLDED POLYPEPTIDE-CHAINS; SINGLE-MOLECULE LEVEL; INTRAMOLECULAR CONTACT FORMATION; RESONANCE ENERGY-TRANSFER; DOUBLE-STRANDED DNA; CORRELATION SPECTROSCOPY; CHARGE-TRANSFER; REDUCTION POTENTIALS; DISORDERED PROTEINS; REDOX POTENTIALS;

D O I：

10.1002/cphc.200900238

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Photoinduced electron transfer (PET) between organic fluorophores and suitable electron donating moieties, for example, the amino acid tryptophan or the nucleobase guanine, can quench fluorescence upon van der Waals contact and thus report-on molecular contact. PET-quenching has been used as reporter for monitoring conformational dynamics in polypeptides, proteins, and oligonucleotides. Whereas dynamic quenching transiently influences quantum yield and fluorescence life-time of the fluorophore, static quenching in pi-stacked complexes efficiently suppresses fluorescence emission over time scales longer than the fluorescence lifetime. Static quenching therefore provides sufficient contrast to be observed at the single-molecule level. Here, we review complex formation and static quenching of different fluorophores by various molecular compounds, discuss applications as reporter system for macromolecular dynamics, and give illustrating examples.

引用

页码：1389 / 1398

页数：10

共 100 条

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