Carbon nanotubes promote neuron differentiation from human embryonic stem cells

被引：144

作者：

Chao, Tzu-I ^{[1
]}

Xiang, Shuhuai ^{[1
]}

Chen, Chi-Shuo ^{[1
]}

Chin, Wei-Chun ^{[1
]}

Nelson, A. J. ^{[2
]}

Wang, Changchun ^{[3
,4
]}

Lu, Jennifer ^{[1
]}

机构：

[1] Univ Calif, UC Merced, Sch Engn, Merced, CA 95343 USA

[2] Lawrence Livermore Natl Lab, Phys & Life Sci Directorate, Livermore, CA 94550 USA

[3] Fudan Univ, Key Lab Mol Engn Polymers, Minist Educ, Dept Macromol Sci, Shanghai 200433, Peoples R China

[4] Fudan Univ, Adv Mat Lab, Shanghai 200433, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 384卷 / 04期

关键词：

Human embryonic stem cell; Differentiation; Carbon nanotube; Scaffold; Polymer; Poly(acrylic) acid; SURFACES; MATRIX; FUNCTIONALIZATION; ADHESION; DENSITY; LAMININ; GROWTH;

D O I：

10.1016/j.bbrc.2009.04.157

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human embryonic stem cells (hESCs) hold great promise for regenerative medicine and transplantation therapy due to their self-renewal and pluripotent properties. We report that 2D thin film scaffolds composed of biocompatible polymer grafted carbon nanotubes (CNTs), can selectively differentiate human embryonic stem cells into neuron cells while maintaining excellent cell viability. According to fluorescence image analysis, neuron differentiation efficiency of poly(acrylic acid) grafted CNT thin films is significant greater than that on poly(acrylic acid) thin films. When compared with the conventional Poly-L-ornithine surfaces, a standard substratum commonly used for neuron culture, this new type thin film scaffold shows enhanced neuron differentiation. No noticeable cytotoxic effect difference has been detected between these two surfaces. The surface analysis and cell adhesion study have suggested that CNT-based surfaces can enhance protein adsorption and cell attachment. This finding indicates that CNT-based materials are excellent candidates for hESCs' neuron differentiation. Published by Elsevier Inc

引用

页码：426 / 430

页数：5

共 40 条

[1] Biosensors based on carbon nanotubes [J].

Balasubramanian, Kannan ;

Burghard, Marko .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2006, 385 (03) :452-468

[2] Polymer-grafted multiwalled carbon nanotubes through surface-initiated polymerization [J].

Baskaran, D ;

Mays, JW ;

Bratcher, MS .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (16) :2138-2142

[3] Side-wall functionalization of single-walled carbon nanotubes with 4-hydroxymethylaniline followed by polymerization of ε-caprolactone [J].

Buffa, F ;

Hu, H ;

Resasco, DE .

MACROMOLECULES, 2005, 38 (20) :8258-8263

[4]

Cellot G, 2009, NAT NANOTECHNOL, V4, P126, DOI [10.1038/NNANO.2008.374, 10.1038/nnano.2008.374]

[5] Cell-cell and cell-extracellular matrix interactions regulate embryonic stem cell differentiation [J].

Chen, Silvia S. ;

Fitzgerald, Wendy ;

Zimmerberg, Joshua ;

Kleinman, Hynda K. ;

Margolis, Leonid .

STEM CELLS, 2007, 25 (03) :553-561

[6] The control of human mesenchymal cell differentiation using nanoscale symmetry and disorder [J].

Dalby, Matthew J. ;

Gadegaard, Nikolaj ;

Tare, Rahul ;

Andar, Abhay ;

Riehle, Mathis O. ;

Herzyk, Pawel ;

Wilkinson, Chris D. W. ;

Oreffo, Richard O. C. .

NATURE MATERIALS, 2007, 6 (12) :997-1003

[7] A rough morphology of the adsorbed fibronectin layer favors adhesion of neuronal cells [J].

Dekeyser, C. M. ;

Zuyderhoff, E. ;

Giuliano, R. E. ;

Federoff, H. J. ;

Dupont-Gillain, Ch. C. ;

Rouxhet, P. G. .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2008, 87A (01) :116-128

[8] Adhesion of mammalian cells to polymer surfaces: from physical chemistry of surfaces to selective adhesion on defined patterns [J].

Dewez, JL ;

Lhoest, JB ;

Detrait, E ;

Berger, V ;

Dupont-Gillain, CC ;

Vincent, LM ;

Schneider, YJ ;

Bertrand, P ;

Rouxhet, PG .

BIOMATERIALS, 1998, 19 (16) :1441-1445

[9] Matrix elasticity directs stem cell lineage specification [J].

Engler, Adam J. ;

Sen, Shamik ;

Sweeney, H. Lee ;

Discher, Dennis E. .

CELL, 2006, 126 (04) :677-689

[10] Human Embryonic Stem Cell Differentiation Toward Regional Specific Neural Precursors [J].

Erceg, Slaven ;

Ronaghi, Mohammad ;

Stojkovic, Miodrag .

STEM CELLS, 2009, 27 (01) :78-87

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