Validation of a gas-chromatographic method for volatile amine determination in fish samples

被引：43

作者：

VecianaNogues, MT

AlbalaHurtado, MS

IzquierdoPulido, M

VidalCarou, MC

机构：

[1] U. de Nutr. y Bromatologia, Facultad de Farmacia, Universidad de Barcelona, 08028, Barcelona, Av. Joan XXIII, s/n

来源：

FOOD CHEMISTRY | 1996年 / 57卷 / 04期

关键词：

D O I：

10.1016/S0308-8146(96)00031-3

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A gas-chromatographic (GC) procedure is described for the determination of volatile amines in fish. The method includes a first step of volatile amine extraction with 0.6 N perchloric acid, followed by alkalinization with 65% (w/w) KOH and a subsequent toluene extraction. Dimethylamine (DMA) and trimethylamine (TMA) were well resolved; moreover, monomethylamine (MMA) did not interfere in the determination of DMA and TMA, and can also be useful in assessing spoilage of crustaceans. The suitability of toluene as a solvent for extraction is demonstrated. Linearity, precision and recovery were satisfactory. Sensitivity limits were lower than 0.038, 0.110 and 0.167 mg N per 100 g for TMA, DMA and MMA, respectively. In addition, contents of DMA and TMA in fresh and frozen hake samples were studied. Copyright (C) 1996 Elsevier Science Ltd

引用

页码：569 / 573

页数：5

共 21 条

[1]

[Anonymous], 1982, ANAL CHEM, DOI DOI 10.1021/AC00238A765

[2] PRODUCTION OF DIMETHYLAMINE IN MUSCLE OF SEVERAL SPECIES OF GADOID FISH DURING FROZEN STORAGE, ESPECIALLY IN RELATION TO PRESENCE OF DARK MUSCLE [J].

CASTELL, CH ;

SMITH, B ;

NEAL, W .

JOURNAL OF THE FISHERIES RESEARCH BOARD OF CANADA, 1971, 28 (01) :1-&

[3] GRADING FISH FOR QUALITY .1. TRIMETHYLAMINE VALUES OF FILLETS CUT FROM GRADED FISH [J].

CASTELL, CH ;

GREENOUGH, MF ;

RODGERS, RS ;

MACFARLANE, AS .

JOURNAL OF THE FISHERIES RESEARCH BOARD OF CANADA, 1958, 15 (04) :701-716

[4]

Dyer W. J., 1945, JOUR FISH RES BD CANADA, V6, P359

[5]

Dyer W. J., 1945, JOUR FISH RES BD CANADA, V6, P351

[6]

FIDDLER W, 1991, J ASSOC OFF ANA CHEM, V74, P400

[7]

GALLARDO JM, 1991, REV AGROQUIM TECNOL, V31, P103

[8]

HUSS HH, 1988, PESCADO FRESCO SU CA

[9]

KRUSE R, 1989, ARCH LEBENSMITTELHYG, V40, P87

[10] FEASIBILITY STUDY ON THE DETERMINATION OF FISH FRESHNESS BY TRIMETHYLAMINE HEADSPACE ANALYSIS [J].

KRZYMIEN, ME ;

ELIAS, L .

JOURNAL OF FOOD SCIENCE, 1990, 55 (05) :1228-1232

← 1 2 3 →