A fused gene of nucleoprotein (NIP) and herpes simplex virus genes (VP22) induces highly protective immunity against different subtypes of influenza virus

被引：50

作者：

Saha, Sukumar ^{[1
]}

Yoshida, Shinsuke ^{[1
]}

Ohba, Kenji ^{[1
]}

Matsui, Kiyohiko ^{[1
]}

Matsuda, Tomoko ^{[1
]}

Takeshita, Fumihiko ^{[1
]}

Umeda, Kazunori ^{[1
]}

Tamura, Yuichi ^{[1
]}

Okuda, Kentaro ^{[3
]}

Klinman, Dennis ^{[2
]}

Xin, Ke-Qin ^{[1
]}

Okuda, Kenji ^{[1
]}

机构：

[1] Yokohama City Univ, Grad Sch Med, Dept Mol Biodef Res, Kanazawa Ku, 3-9 Fukuura, Yokohama, Kanagawa 2360004, Japan

[2] US FDA, CBER, Bethesda, MD 20892 USA

[3] Kyushu Univ, Fac Med Sci, Dept Oral Hlth Technol & Epidemiol, Fukuoka 81285822, Japan

来源：

VIROLOGY | 2006年 / 354卷 / 01期

关键词：

influenza nucleoprotein (NP); herpes simplex virus gene protein 22 (VP22); DNA vaccine; A/PR/8/34; HSV-1; CD8(+) cells; CD4(+) cells; intercellular spreading;

D O I：

10.1016/j.virol.2006.04.015

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We evaluated the immunogenicity and protective activity of plasmid DNA vaccines encoding the influenza virus NP gene (pNP) alone or in combination with the herpes simplex virus type 1 protein 22 gene (pVP22). Optimal immune responses were observed in BALB/c mice immunized with the combination of pVP22 plus pNP, as assessed by enzyme-linked immunosorbent assay (ELISA), enzyme-linked immunospot (ELISPOT) and intracellular cytokine staining (ICCS). These mice also showed maximal resistance following challenge with the A/PR/8/34 (H1N1) and A/Udron/72 (H3N2) strains of influenza virus. The susceptibility of immunized mice to virus infection was significantly increased following depletion of either CD4(+) or CD8(+) T cells. These results indicate that a plasmid DNA vaccine encoding pVP22 plus NP induces a high level of cross-protective immunity against influenza virus subtypes. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：48 / 57

页数：10

共 43 条

[1] Intracellular localization of proteasomal degradation of a viral antigen [J].

Antón, LC ;

Schubert, U ;

Bacík, I ;

Princiotta, MF ;

Wearsch, PA ;

Gibbs, J ;

Day, PM ;

Realini, C ;

Rechsteiner, MC ;

Bennink, JR ;

Yewdell, JW .

JOURNAL OF CELL BIOLOGY, 1999, 146 (01) :113-124

[2] Electroporation improves the efficacy of DNA vaccines in large animals [J].

Babiuk, S ;

Baca-Estrada, ME ;

Foldvari, M ;

Storms, M ;

Rabussay, D ;

Widera, G ;

Babiuk, LA .

VACCINE, 2002, 20 (27-28) :3399-3408

[3] Influenza virus nucleoprotein interacts with influenza virus polymerase proteins [J].

Biswas, SK ;

Boutz, PL ;

Nayak, DP .

JOURNAL OF VIROLOGY, 1998, 72 (07) :5493-5501

[4] ENHANCED RECOGNITION OF A MODIFIED PEPTIDE ANTIGEN BY CYTOTOXIC-T CELLS SPECIFIC FOR INFLUENZA NUCLEOPROTEIN [J].

BODMER, HC ;

PEMBERTON, RM ;

ROTHBARD, JB ;

ASKONAS, BA .

CELL, 1988, 52 (02) :253-258

[5] DNA immunization of newborn mice with a plasmid-expressing nucleoprotein of influenza virus [J].

Bot, A ;

Bot, S ;

GarciaSastre, A ;

Bona, C .

VIRAL IMMUNOLOGY, 1996, 9 (04) :207-210

[6] Particle assembly incorporating a VP22-BH3 fusion protein, facilitating intracellular delivery, regulated release, and apoptosis [J].

Brewis, ND ;

Phelan, A ;

Normand, N ;

Choolun, E ;

O'Hare, P .

MOLECULAR THERAPY, 2003, 7 (02) :262-270

[7] Several protein regions contribute to determine the nuclear and cytoplasmic localization of the influenza A virus nucleoprotein [J].

Bullido, R ;

Gómez-Puertas, P ;

Albo, C ;

Portela, A .

JOURNAL OF GENERAL VIROLOGY, 2000, 81 :135-142

[8] Protection and antibody responses in different strains of mouse immunized with plasmid DNAs encoding influenza virus haemagglutinin, neuraminidase and nucleoprotein [J].

Chen, Z ;

Yoshikawa, T ;

Kadowaki, S ;

Hagiwara, Y ;

Matsuo, K ;

Asanuma, H ;

Aizawa, C ;

Kurata, T ;

Tamura, S .

JOURNAL OF GENERAL VIROLOGY, 1999, 80 :2559-2564

[9] Non-lethal viral challenge of influenza haemagglutinin and nucleoprotein DNA vaccinated mice results in reduced viral replication [J].

Cox, RJ ;

Mykkeltvedt, E ;

Robertson, J ;

Haaheim, LR .

SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2002, 55 (01) :14-23

[10]

DIALYNAS DP, 1983, J IMMUNOL, V131, P2445

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