A fused gene of nucleoprotein (NIP) and herpes simplex virus genes (VP22) induces highly protective immunity against different subtypes of influenza virus

被引：50

作者：

Saha, Sukumar ^{[1
]}

Yoshida, Shinsuke ^{[1
]}

Ohba, Kenji ^{[1
]}

Matsui, Kiyohiko ^{[1
]}

Matsuda, Tomoko ^{[1
]}

Takeshita, Fumihiko ^{[1
]}

Umeda, Kazunori ^{[1
]}

Tamura, Yuichi ^{[1
]}

Okuda, Kentaro ^{[3
]}

Klinman, Dennis ^{[2
]}

Xin, Ke-Qin ^{[1
]}

Okuda, Kenji ^{[1
]}

机构：

[1] Yokohama City Univ, Grad Sch Med, Dept Mol Biodef Res, Kanazawa Ku, 3-9 Fukuura, Yokohama, Kanagawa 2360004, Japan

[2] US FDA, CBER, Bethesda, MD 20892 USA

[3] Kyushu Univ, Fac Med Sci, Dept Oral Hlth Technol & Epidemiol, Fukuoka 81285822, Japan

来源：

VIROLOGY | 2006年 / 354卷 / 01期

关键词：

influenza nucleoprotein (NP); herpes simplex virus gene protein 22 (VP22); DNA vaccine; A/PR/8/34; HSV-1; CD8(+) cells; CD4(+) cells; intercellular spreading;

D O I：

10.1016/j.virol.2006.04.015

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We evaluated the immunogenicity and protective activity of plasmid DNA vaccines encoding the influenza virus NP gene (pNP) alone or in combination with the herpes simplex virus type 1 protein 22 gene (pVP22). Optimal immune responses were observed in BALB/c mice immunized with the combination of pVP22 plus pNP, as assessed by enzyme-linked immunosorbent assay (ELISA), enzyme-linked immunospot (ELISPOT) and intracellular cytokine staining (ICCS). These mice also showed maximal resistance following challenge with the A/PR/8/34 (H1N1) and A/Udron/72 (H3N2) strains of influenza virus. The susceptibility of immunized mice to virus infection was significantly increased following depletion of either CD4(+) or CD8(+) T cells. These results indicate that a plasmid DNA vaccine encoding pVP22 plus NP induces a high level of cross-protective immunity against influenza virus subtypes. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：48 / 57

页数：10

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