PROTECTIVE IMMUNITY BY INTRAMUSCULAR INJECTION OF LOW-DOSES OF INFLUENZA-VIRUS DNA VACCINES

被引：152

作者：

ULMER, JB ^{[1
]}

DECK, RR ^{[1
]}

DEWITT, CM ^{[1
]}

FRIEDMAN, A ^{[1
]}

DONNELLY, JJ ^{[1
]}

LIU, MA ^{[1
]}

机构：

[1] MERCK SHARP & DOHME LTD,RES LABS,DEPT VIRUS & CELL BIOL,W POINT,PA 19486

来源：

VACCINE | 1994年 / 12卷 / 16期

关键词：

INFLUENZA; DOSE-RESPONSE; DNA VACCINE;

D O I：

10.1016/0264-410X(94)90081-7

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Dose-response relationships were investigated between dose of influenza virus haemagglutinin (HA) or nucleoprotein (NP) DNA vaccines, and immunogenicity and protective efficacy based on humoral and cellular immunity. In mice, intramuscular (i.m.) injection of HA or NP DNA, at doses of 100 ng to 1 mu g, was found to generate haemagglutination inhibiting (HI) antibodies and cytotoxic T-lymphocytes, respectively, and provide protection in influenza virus challenge models. A direct correlation between the amount of DNA injected and the level of HI antibody was observed. In non-human primates, high-titre neutralizing antibodies were induced in animals vaccinated with as little as 10 mu g of HA DNA. These results indicate that low doses of DNA administered by i.m. injection provide protective efficacy against influenza.

引用

页码：1541 / 1544

页数：4

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