Miniaturized separation techniques in glycomic investigations

被引：49

作者：

Mechref, Yehia ^{[1
]}

Novotny, Milos V. ^{[1
]}

机构：

[1] Indiana Univ, Natl Ctr Glycom & Glycoproteom, Dept Chem, Bloomington, IN 47405 USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 841卷 / 1-2期

关键词：

glycomics; oligosaccharides; capillary liquid chromatography; capillary electrophoresis; capillary electrochromatography; hydrophilic nano-LC; graphitized carbon packings; permethylation;

D O I：

10.1016/j.jchromb.2006.04.049

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High-sensitivity glycomic analyses are becoming of a great interest in modern biomedical and clinical research, as well as in the development of recombinant protein products. The evolution of separation techniques for glycomic analysis at high sensitivity is highlighted in this review. These methodologies include capillary liquid chromatography, capillary electrophoresis (CE) and capillary electrochromatography (CEC). The potential of such methodologies in glycomic analysis is demonstrated for model glycoproteins as well as total glycomes derived from biological samples. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：65 / 78

页数：14

共 143 条

[1] HYDROPHILIC-INTERACTION CHROMATOGRAPHY FOR THE SEPARATION OF PEPTIDES, NUCLEIC-ACIDS AND OTHER POLAR COMPOUNDS [J].