Plastid transformation in higher plants

被引：311

作者：

Maliga, P

机构：

[1] Rutgers State Univ, Waksman Inst, Piscataway, NJ 08854 USA

[2] Rutgers State Univ, Dept Plant Biol, New Brunswick, NJ 08901 USA

来源：

ANNUAL REVIEW OF PLANT BIOLOGY | 2004年 / 55卷

关键词：

plastid genetics; plastid markers; protein expression; gene knockouts; gene containment;

D O I：

10.1146/annurev.arplant.55.031903.141633

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Plastids of higher plants are semi-autonomous organelles with a small, highly polyploid genome and their own transcription-translation machinery. This review provides an overview of the technology for the genetic modification of the plastid genome including: vectors, marker genes and gene design, the use of gene knockouts and over-expression to probe plastid function and the application of site-specific recombinases for excision of target DNA. Examples for applications in basic science include the study of plastid gene transcription, mRNA editing, photosynthesis and evolution. Examples for biotechnological applications are incorporation of transgenes in the plastid genome for containment and high-level expression of recombinant proteins for pharmaceutical and industrial applications. Plastid transformation is routine only in tobacco. Progress in implementing the technology in other crops is discussed.

引用

页码：289 / 313

页数：29

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