Differential stimulation of monocytic cells results in distinct populations of microparticles

被引：131

作者：

Bernimoulin, M. ^{[1
,2
]}

Waters, E. K. ^{[1
,2
]}

Foy, M. ^{[1
,2
,3
]}

Steele, B. M. ^{[3
]}

Sullivan, M. ^{[3
]}

Falet, H. ^{[4
]}

Walsh, M. T. ^{[1
]}

Barteneva, N. ^{[1
]}

Geng, J-G. ^{[5
]}

Hartwig, J. H. ^{[4
]}

Maguire, P. B. ^{[3
]}

Wagner, D. D. ^{[1
,2
]}

机构：

[1] Immune Dis Inst, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA

[3] Univ Coll Dublin, Sch Biomol & Biomed Sci, UCD Conway Inst, Dublin 4, Ireland

[4] Brigham & Womens Hosp, Translat Med Div, Boston, MA 02115 USA

[5] Univ Minnesota, Sch Med, Vasc Biol Ctr, Div Hematol Oncol & Transplantat,Dept Med, Minneapolis, MN 55455 USA

来源：

JOURNAL OF THROMBOSIS AND HAEMOSTASIS | 2009年 / 7卷 / 06期

基金：

美国国家卫生研究院; 瑞士国家科学基金会;

关键词：

LPS; microparticles; monocytes; phosphatidylserine; proteomics; P-selectin; P-SELECTIN; TISSUE FACTOR; MEMBRANE MICROPARTICLES; ACTIVATED PLATELETS; FLUORESCENCE ASSAY; ENDOTHELIAL-CELLS; IN-VIVO; RECEPTOR; PROTEIN; EXPRESSION;

D O I：

10.1111/j.1538-7836.2009.03434.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background: Microparticles (MPs), small vesicles shed from stimulated cells, permit cross-talk between cells within a particular environment. Their composition is thought to reflect their cell of origin, and differs according to whether they are produced by stimulation or by apoptosis. Whether MP properties vary according to stimulus is not yet known. Methods: We studied the characteristics of MPs produced from monocytic THP-1 cells upon stimulation with lipopolysaccharide or a soluble P-selectin chimera, using proteomics, flow cytometry, western blotting, and electron microscopy. Results: Utilizing a novel criterion of calcein-AM staining to define MPs, we found that MP populations were similar with respect to size, presence and organization of cytoskeleton, and expression of certain antigens. The MPs shared the same level of procoagulant activity. We found that MPs also have distinct characteristics, depending on stimuli. These include differences in phosphatidylserine expression and expression of proteins from specific subcellular locations such as the mitochondria, and of unique antigens such as leukocyte-associated immunoglobin-like-receptor (LAIR)-1, which was found only upon stimulation with the soluble P-selectin chimera. Conclusion: We found that the properties of MPs depend on the stimulus that produced them. This supports the concept that monocytic MPs differentially modulate thrombosis, inflammation and immune regulation according to stimulus.

引用

页码：1019 / 1028

页数：10

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