Chitosan-based DNA delivery vector targeted to gonadotropin-releasing hormone (GnRH) receptor

被引：15

作者：

Boonthum, Chatwalee ^{[1
]}

Namdee, Katawut ^{[2
]}

Boonrungsiman, Suwimon ^{[2
]}

Chatdarong, Kaywalee ^{[1
]}

Saengkrit, Nattika ^{[2
]}

Sajomsang, Warayuth ^{[2
]}

Ponglowhapan, Suppawiwat ^{[1
]}

Yata, Teerapong ^{[2
]}

机构：

[1] Chulalongkorn Univ, Fac Vet Sci, Dept Obstet Gynaecol & Reprod, Bangkok 10330, Thailand

[2] Natl Sci & Technol Dev Agcy, Natl Nanotechnol Ctr NANOTEC, 111 Thailand Sci Pk,Paholyothin Rd, Klongluang 12120, Pathum Thani, Thailand

来源：

CARBOHYDRATE POLYMERS | 2017年 / 157卷

关键词：

Gonadotropin releasing hormone (GnRH); Chitosan; Gene delivery; IN-VITRO CYTOTOXICITY; GENE DELIVERY; CELL BIOLOGY; CARRIER; CANCER; POLYMERS; THERAPY; SYSTEM; LINE;

D O I：

10.1016/j.carbpol.2016.09.015

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

The main purpose of this study was to investigate the application of modified chitosan as a potential vector for gene delivery to gonadotropin-releasing hormone receptor (GnRHR)-expressing cells. Such design of gene carrier could be useful in particular for gene therapy for cancers related to the reproductive system, gene disorders of sexual development, and contraception and fertility control. In this study, a decapeptide GnRH was successfully conjugated to chitosan (CS) as confirmed by proton nuclear magnetic resonance spectroscopy (H-1 NMR) and Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The synthesized GnRH-conjugated chitosan (GnRH-CS) was able to condense DNA to form positively charged nanoparticles and specifically deliver plasmid DNA to targeted cells in both two-dimensional (2D) and three-dimensional (3D) cell cultures systems. Importantly, GnRH-CS exhibited higher transfection activity compared to unmodified CS. In conclusion, GnRH-conjugated chitosan can be a promising carrier for targeted DNA delivery to GnRHR-expressing cells. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：311 / 320

页数：10

共 31 条

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Expression of G protein-coupled receptors and related proteins in HEK293, AtT20, BV2, and N18 cell lines as revealed by microarray analysis [J].