Disposable Strip Biosensor for Visual Detection of Hg2+ Based on Hg2+-Triggered Toehold Binding and Exonuclease III-Assisted Signal Amplification

被引：150

作者：

Chen, Junhua ^{[1
]}

Zhou, Shungui ^{[1
]}

Wen, Junlin ^{[1
]}

机构：

[1] Guangdong Inst Ecoenvironm & Soil Sci, Guangzhou 510650, Guangdong, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2014年 / 86卷 / 06期

关键词：

CRYSTAL MICROBALANCE BIOSENSOR; DNA DETECTION; COLORIMETRIC DETECTION; GOLD NANOPARTICLES; QUANTUM DOTS; IONS; SENSOR; MERCURY(II); BIOMARKER; EXPOSURE;

D O I：

10.1021/ac404170j

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

A disposable strip biosensor for the visual detection of Hg2+ in aqueous solution has been constructed on the basis of Hg2+-triggered toehold binding and exonuclease III (Exo III)-assisted signal amplification. Thymine-thymine (T-T) mismatches in the toehold domains can serve as specific recognition elements for Hg2+. binding with the help of T-Hg2+-T base pairs to initiate toehold-mediated strand displacement reaction. Exo III-catalyzed target recycling strategy is introduced to improve the sensitivity. Using gold nanoparticles as a tracer, the output signals can be directly observed by the naked eye. The assay is ultrasensitive, enabling the visual detection of trace amounts of Hg2+ as low as I pM without instrumentation. This sensing system also displays remarkable specificity to Hg2+ against other possible competing ions. This sensor is robust and can be applied to the reliable monitoring of spiked Hg2+ in environmental water samples with good recovery and accuracy. With the advantages of cost-effectiveness, simplicity, portability, and convenience, the disposable strip biosensor will be a promising candidate for point-of-use monitoring of Hg2+ in environmental and biological samples.

引用

页码：3108 / 3114

页数：7

共 40 条

[1]

Enzyme-free strip biosensor for amplified detection of Pb2+ based on a catalytic DNA circuit [J].

Chen, Junhua ;

Zhou, Xuemeng ;

Zeng, Lingwen .

CHEMICAL COMMUNICATIONS, 2013, 49 (10) :984-986

[2]

Computational Lateral Flow Biosensor for Proteins and Small Molecules: A New Class of Strip Logic Gates [J].

Chen, Junhua ;

Fang, Zhiyuan ;

Lie, Puchang ;

Zeng, Lingwen .

ANALYTICAL CHEMISTRY, 2012, 84 (15) :6321-6325

[3]

DNA-metal base pairs [J].

Clever, Guido H. ;

Kaul, Corinna ;

Carell, Thomas .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2007, 46 (33) :6226-6236

[4]

Metallo-toeholds: controlling DNA strand displacement driven by Hg(II) ions [J].

Ding, Wei ;

Deng, Wei ;

Zhu, Hong ;

Liang, Haojun .

CHEMICAL COMMUNICATIONS, 2013, 49 (85) :9953-9955

[5]

Integrated Lateral Flow Test Strip with Electrochemical Sensor for Quantification of Phosphorylated Cholinesterase: Biomarker of Exposure to Organophosphorus Agents [J].

Du, Dan ;

Wang, Jun ;

Wang, Limin ;

Lu, Donglai ;

Lin, Yuehe .

ANALYTICAL CHEMISTRY, 2012, 84 (03) :1380-1385

[6]

Amplified Multiplexed Analysis of DNA by the Exonuclease III-Catalyzed Regeneration of the Target DNA in the Presence of Functionalized Semiconductor Quantum Dots [J].

Freeman, Ronit ;

Liu, Xiaoqing ;

Willner, Itamar .

NANO LETTERS, 2011, 11 (10) :4456-4461

[7]

Voltammetric determination of mercury(II) [J].

Gao, Chao ;

Huang, Xing-Jiu .

TRAC-TRENDS IN ANALYTICAL CHEMISTRY, 2013, 51 :1-12

[8]

Amplified colorimetric detection of mercuric ions through autonomous assembly of G-quadruplex DNAzyme nanowires [J].

Hao, Yanli ;

Guo, Qingquan ;

Wu, Hanyin ;

Guo, Liangqia ;

Zhong, Liangshuang ;

Wang, Jing ;

Lin, Tianran ;

Fu, FengFu ;

Chen, Guonan .

BIOSENSORS & BIOELECTRONICS, 2014, 52 :261-264

[9]

The chemical form of mercury in fish [J].

Harris, HH ;

Pickering, IJ ;

George, GN .

SCIENCE, 2003, 301 (5637) :1203-1203

[10]

Visual detection of gene mutations based on isothermal strand-displacement polymerase reaction and lateral flow strip [J].

He, Yuqing ;

Zeng, Kang ;

Zhang, Sanquan ;

Gurung, Anant S. ;

Baloda, Meenu ;

Zhang, Xibao ;

Liu, Guodong .

BIOSENSORS & BIOELECTRONICS, 2012, 31 (01) :310-315

← 1 2 3 4 →