Photostability of thiol-capped CdTe quantum dots in living cells: the effect of photo-oxidation

被引：78

作者：

J Ma

Ji-Yao Chen ^{[1
]}

J Guo

C C Wang

W L Yang

L Xu

P N Wang

机构：

[1] Fudan Univ, Dept Phys, Shanghai 200433, Peoples R China

[2] Fudan Univ, State Key Lab Appl Surface Phys, Shanghai 200433, Peoples R China

[3] Fudan Univ, State Key Lab Adv Photon Mat & Devices, Shanghai 200433, Peoples R China

[4] Fudan Univ, Dept Macromol Sci, Shanghai 200433, Peoples R China

[5] Fudan Univ, Key Lab Mol Engn Polymers, Shanghai 200433, Peoples R China

来源：

NANOTECHNOLOGY | 2006年 / 17卷 / 09期

关键词：

D O I：

10.1088/0957-4484/17/9/002

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The photostability of thiol-capped CdTe quantum dots (QDs) in Euglena gracilis (EG 277) and human embryonic kidney (HEK 293) cells was studied. The photobleaching for the cellular QDs is dependent both on the irradiation power density and the QD local concentration. The photostability of cellular QDs is better than that of chlorophyll in EG 277 cells and of green fluorescence protein (GFP) in HEK 293 cells, and is much better than that of FITC when the local concentration of QDs is not too low. The photobleaching of cellular QDs was remarkably reduced in the nitrogen treated EG 277 cells, indicating that photobleaching in living cells mainly results from photo-oxidation. The effect of photo-oxidation on QD photobleaching was further confirmed by comparing the situations in oxygen treated and nitrogen treated QD aqueous solutions. The photobleaching rate is related to the irradiation power density and the local density of QDs. The higher irradiation power density and oxygen abundance and lower QD concentration will result in a higher photobleaching rate.

引用

页码：2083 / 2089

页数：7

共 33 条

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