Induction of apoptosis in purified animal and plant nuclei by Xenopus egg extracts

被引:5
作者
Jiang, ZF [1 ]
Zhu, S [1 ]
Sun, YL [1 ]
Zhai, ZH [1 ]
机构
[1] Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China
关键词
apoptosis; Xenopus laevis; cell-free; carrot; mouse; dATP;
D O I
10.1038/sj.cr.7290008
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We have developed a cell-free system that can trigger the nuclei purified from mouse liver and suspension-cultured carrot cells to undergo apoptosis as defined by the formation of apoptotic bodies and nucleosomal DNA fragments. The effects of different divalent cations and cycloheximide on DNA cleavage in this system were assessed. The fact that nuclei of plant cells can be induced to undergo apoptosis in a cell-free animal system suggests that animals and plants share a common signal transduction pathway triggering in the initiation stage of apoptosis.
引用
收藏
页码:79 / 90
页数:12
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