Adeno-associated virus Rep78 binds to E2-responsive element 1 of bovine papillomavirus type 1

被引:2
作者
Chon, SK
Rim, BM
Im, DS
机构
[1] Chonbuk Natl Univ, Coll Vet Med, Chonju 560756, South Korea
[2] KIST, Gene Therapy Unit, Korea Res Inst Biosci & Biotechnol, Taejon 305600, South Korea
关键词
adeno-associated virus type-2; bovine papillomavirus type-1; E2-responsive element 1; Rep78;
D O I
10.1080/152165499306766
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Adeno-associated virus type-2 (AAV-2) is a helper-dependent parvovirus that has been implicated in the inhibition of replication and oncogenic transformation of bovine papillomavirus type-1 (BPV-I) and other transforming DNA viruses. Previous studies have suggested that the Rep78 protein of AAV-2 is a key player mediating this effect. In this report we have analyzed the effect of AAV-2 Rep78 protein on the regulation of gene expression of a reporter gene under the control of the long control region (LCR) of BPV-1. Our results show that Rep78 is capable of down-regulating the promoter activity of the the LCR in vivo in tissue culture cells. Inhibition of LCR activity in vivo suggested the need for Rep78 to bind to a region of the LCR promoter spanning the E2-responsive elements of BPV-1, This observation was further confirmed in vitro with gel shift assays showing specific binding of Rep78 to DNA oligonucleotides containing E2-responsive element 1 (E2RE(1)) sequences of BPV-1 LCR. Our results expand the understanding of the mechanism of trans-regulation mediated by Rep78 and involving this protein and DNA sequences with complex secondary structure.
引用
收藏
页码:397 / 404
页数:8
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