Negative regulation of the mammalian UV response by Myc through association with Miz-1

被引：257

作者：

Herold, S

Wanzel, M

Beuger, V

Frohme, C

Beul, D

Hillukkala, T

Syvaoja, J

Saluz, HP

Haenel, F

Eilers, M ^{[1
]}

机构：

[1] Inst Mol Biol & Tumor Res, D-35033 Marburg, Germany

[2] Univ Oulu, Dept Biochem, FIN-90014 Oulu, Finland

[3] Bioctr Oulu, FIN-90014 Oulu, Finland

[4] Hans Knoll Inst Nat Stoff Forsch, Dept Cell & Mol Biol, D-07745 Jena, Germany

来源：

MOLECULAR CELL | 2002年 / 10卷 / 03期

基金：

芬兰科学院;

关键词：

D O I：

10.1016/S1097-2765(02)00633-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Myc oncoprotein represses initiator-dependent transcription through the POZ domain transcription factor Miz-1. We now show that transactivation by Miz-1 is negatively regulated by association with topoisomerase II binding protein (TopBP1); UV irradiation downregulates expression of TopBP1 and releases Miz-1. Miz-1 binds to the p21Cip1 core promoter in vivo and is required for upregulation of p21Cip1 upon UV irradiation. Using both c-myc(-/-) cells and a point mutant of Myc that is deficient in Miz-1 dependent repression, we show that Myc negatively regulates transcription of p21Cip1 upon UV irradiation and facilitates recovery from UV-induced cell cycle arrest through binding to Miz-1. Our data implicate Miz-1 in a pathway that regulates cell proliferation in response to UV irradiation.

引用

页码：509 / 521

页数：13

共 32 条

[1] THE C-MYC PROTEIN INDUCES CELL-CYCLE PROGRESSION AND APOPTOSIS THROUGH DIMERIZATION WITH MAX [J].