Binding of NF-Y transcription factor to one of the cis-elements in the myeloperoxidase gene promoter that responds to granulocyte colony-stimulating factor

被引:23
作者
Orita, T
Shimozaki, K
Murakami, H
Nagata, S
机构
[1] OSAKA UNIV, SCH MED, DEPT GENET, SUITA, OSAKA 565, JAPAN
[2] OSAKA BIOSCI INST, SUITA, OSAKA 565, JAPAN
关键词
D O I
10.1074/jbc.272.37.23216
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The expression of the myeloperoxidase (MPO) gene is restricted to cells of the myeloid cell lineage and is induced by granulocyte colony stimulating factor (G-CSF). In this study, a series of deletion mutations was introduced in the promoter of the human MPO gene, which was then fused to the chloramphenicol acetyltransferase gene. The G-CSF-induced promoter activity was examined in mouse myeloid precursor FDC-P1 transformants that constitutively express the G-CSF receptor. A G-CSF-responsive element (GRE) in the MPO gene was found approximately 800 base pairs upstream from the transcription initiation site. When the 5'-flanking region of the human MPO gene contained this element, it yielded promoter activity in cells cultured with G-CSF but not in cells cultured with interleukin 3. Gel shift assays with the element showed that a specific nuclear factor(s) (NF/G-CSF) binds to the element. The NF/G-CSF was purified by affinity chromatography using an oligonucleotide of GRE. Protein sequence analysis of the purified NF/G-CSF indicated that NF/G-CSF is a ubiquitous transcription factor, NF-Y, which is composed of three subunits. The recombinant, NF-Y was then shown to bind to GRE in a combination of the three subunits.
引用
收藏
页码:23216 / 23223
页数:8
相关论文
共 51 条
  • [21] LIWEBER M, 1994, J IMMUNOL, V153, P4122
  • [22] LUBBERT M, 1991, BLOOD, V78, P345
  • [23] Developmental regulation of myeloid gene expression and demethylation during ex vivo culture of peripheral blood progenitor cells
    Lubbert, M
    Brugger, W
    Mertelsmann, R
    Kanz, L
    [J]. BLOOD, 1996, 87 (02) : 447 - 455
  • [24] MAHONEY CW, 1992, J BIOL CHEM, V267, P19396
  • [25] THE ROLE OF CIS-ACTING PROMOTER ELEMENTS IN TISSUE-SPECIFIC ALBUMIN GENE-EXPRESSION
    MAIRE, P
    WUARIN, J
    SCHIBLER, U
    [J]. SCIENCE, 1989, 244 (4902) : 343 - 346
  • [26] SELECTIVE ACTIVATION OF TRANSCRIPTION BY A NOVEL CCAAT BINDING-FACTOR
    MAITY, SN
    GOLUMBEK, PT
    KARSENTY, G
    DECROMBRUGGHE, B
    [J]. SCIENCE, 1988, 241 (4865) : 582 - 585
  • [27] MAITY SN, 1992, J BIOL CHEM, V267, P16574
  • [28] MONOCLONAL-ANTIBODIES TO NF-Y DEFINE ITS FUNCTION IN MHC CLASS-II AND ALBUMIN GENE-TRANSCRIPTION
    MANTOVANI, R
    PESSARA, U
    TRONCHE, F
    LI, XY
    KNAPP, AM
    PASQUALI, JL
    BENOIST, C
    MATHIS, D
    [J]. EMBO JOURNAL, 1992, 11 (09) : 3315 - 3322
  • [29] ANALYSIS OF TRANSCRIPTIONAL REGULATORY SIGNALS OF THE HSV THYMIDINE KINASE GENE - IDENTIFICATION OF AN UPSTREAM CONTROL REGION
    MCKNIGHT, SL
    GAVIS, ER
    KINGSBURY, R
    AXEL, R
    [J]. CELL, 1981, 25 (02) : 385 - 398
  • [30] CLONING OF YEAST HAP5 - A NOVEL SUBUNIT OF A HETEROTRIMERIC COMPLEX REQUIRED FOR CCAAT BINDING
    MCNABB, DS
    XING, YY
    GUARENTE, L
    [J]. GENES & DEVELOPMENT, 1995, 9 (01) : 47 - 58