Use of a silicone tubing sensor to control methanol concentration during fed batch fermentation of Pichia pastoris

被引：15

作者：

Wagner, LW ^{[1
]}

Matheson, NH ^{[1
]}

Heisey, RF ^{[1
]}

Schneider, K ^{[1
]}

机构：

[1] BIOTECH RESOURCES,LP,MANITOWOC,WI 54220

来源：

BIOTECHNOLOGY TECHNIQUES | 1997年 / 11卷 / 11期

关键词：

D O I：

10.1023/A:1018469007145

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A silicone tubing sensor controlled a constant methanol concentration in a fermenter up to 72 hours without the need for on-line gas chromatography or complex feeding schemes based on dissolved oxygen spikes. Methanol concentration was controlled up to 1.0% (v/v) with control around a given set point of +/-0.24%. The length of tubing, airflow through the tubing, pump speed and medium formulation had no effect on the control of methanol concentration.

引用

页码：791 / 795

页数：5

共 8 条

[1] MONITORING AND CONTROL OF METHANOL CONCENTRATION DURING POLYSACCHARIDE FERMENTATION USING AN ONLINE METHANOL SENSOR [J].