16S-23S rDNA internal transcribed spacer regions in four Proteus species

被引：14

作者：

Cao, Boyang ^{[1
,2
,3
,4
]}

Wang, Min ^{[1
,2
,3
,4
]}

Liu, Lei ^{[1
,2
,3
,4
]}

Zhou, Zhemin ^{[1
,2
,3
,4
]}

Wen, Shaoping ^{[1
,2
,3
,4
]}

Rozalski, Antoni ^{[6
]}

Wang, Lei ^{[1
,2
,3
,4
,5
]}

机构：

[1] Nankai Univ, Sch Biol Sci & Biotechnol, TEDA, Tianjin 300457, Peoples R China

[2] Nankai Univ, Coll Life Sci, Minist Educ, Key Lab Mol Microbiol & Technol, Tianjin 300071, Peoples R China

[3] Nankai Univ, TEDA Coll, Tianjin Res Ctr Funct Genom & Biochips, Tianjin 300457, Peoples R China

[4] Nankai Univ, TEDA Coll, Tianjin State Lab Microbial Funct Genom, Tianjin 300457, Peoples R China

[5] Tianjin Biochip Corp, TEDA, Tianjin 300457, Peoples R China

[6] Univ Lodz, Inst Microbiol & Immunol, Dept Immunobiol Bacteria, PL-90237 Lodz, Poland

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2009年 / 77卷 / 01期

关键词：

Proteus; ITS; Internal transcribed spacer; 16S/23S RIBOSOMAL SPACER; COPY NUMBER; PHYLOGENETIC-RELATIONSHIPS; SEQUENCE ALIGNMENT; 16S; IDENTIFICATION; VULGARIS; STRAINS; CLASSIFICATION; DEFINITION;

D O I：

10.1016/j.mimet.2009.01.024

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteus is a Gram-negative, facultative anaerobic bacterium. In this study, 813 Proteus 165-235 rDNA internal transcribed spacer (ITS) sequences were determined from 46 Proteus strains, including 388 ITS from 22 P. mirabilis strains, 211 ITS from 12 P vulgaris strains, 169 ITS from 10 P. penneri strains, and 45 ITS from 2 P. myxofaciens strains. The Proteus strains carry mainly two types of ITS, ITSGlu (containing tRNAC(Glu) ((UUC)) gene) and ITSIIe+(Ala) (containing tRNA(IIe) ((GAU)) and tRNA(Ala) ((UGC)) gene), and are in the forms of 28 variants with 25 genomic origins. The ITS sequences are a mosaic-like structure consisting of three conservative regions and two variable regions. The nucleotide identity of ITS subtypes in strains of the same species ranges from 96.2% to 100%. The divergence of Proteus ITS divergence was most likely due to intraspecies recombinations or horizontal transfers of sequence blocks. The phylogenetic relationship deduced from the second variable region of ITS sequences of the three facultative human pathogenic species P. mirabilis, P. vulgaris and P. penneri is similar with that based on 165 rDNA sequences, but has higher resolution to differentiate closely related P. vulgaris and P. penneri. This study is the first comprehensive study of ITS in four Proteus species and laid solid foundation for the development of high-throughput technology for quick and accurate identification of the important foodborne and nosocomial pathogens. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：109 / 118

页数：10