Intersubunit allosteric communication mediated by a conserved loop in the MCM helicase

被引：35

作者：

Barry, Elizabeth R. ^{[1
,2
]}

Lovett, Janet E. ^{[3
]}

Costa, Alessandro ^{[1
]}

Lea, Susan M. ^{[1
]}

Bell, Stephen D. ^{[1
]}

机构：

[1] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX2 6NA, England

[2] Hutchison MRC Ctr, MRC Canc Cell Unit, Cambridge CB2 0XZ, England

[3] Univ Oxford, Inorgan Chem Lab, Ctr Adv ESR, Oxford OX1 3QR, England

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 04期

基金：

英国工程与自然科学研究理事会;

关键词：

archaea; DNA replication; AAA plus protein; Sulfolobus; REPLICATIVE HEXAMERIC HELICASE; ARCHAEAL MCM; DNA; MECHANISM; PROTEIN; COMPLEX; TRANSLOCATION; DOMAINS;

D O I：

10.1073/pnas.0809192106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The minichromosome maintenance (MCM) helicase is the presumptive replicative helicase in archaea and eukaryotes. The archaeal homomultimeric MCM has a two-tier structure. One tier contains the AAA+ motor domains of the proteins, and these are the minimal functional helicase domains. The second tier is formed by the N-terminal domains. These domains are not essential for MCM helicase activity but act to enhance the processivity of the helicase. We reveal that a conserved loop facilitates communication between processivity and motor tiers. Interestingly, this allostery seems to be mediated by interactions between, rather than within, individual protomers in the MCM ring.

引用

页码：1051 / 1056

页数：6

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