Novel transcripts of Nox1 are regulated by alternative promoters and expressed under phenotypic modulation of vascular smooth muscle cells

被引:24
作者
Arakawa, Noriaki
Katsuyama, Masato
Matsuno, Kuniharu
Urao, Norifumi
Tabuchi, Yoshiaki
Okigaki, Mitsuhiko
Matsubara, Hiroaki
Yabe-Nishimura, Chihiro [1 ]
机构
[1] Kyoto Prefectural Univ Med, Dept Pharmacol, Kyoto 6028566, Japan
[2] Kyoto Prefectural Univ Med, Dept Cardiovasc Med, Kyoto 6028566, Japan
[3] Toyama Univ, Life Sci Res Ctr, Div Mol Genet Res, Toyama 9300194, Japan
关键词
alternative promoters; colon epithelial cell (CEC); NADPH oxidase; NOX1; phenotypic modulation; vascular smooth muscle cell (VSMC);
D O I
10.1042/BJ20060300
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
NADPH oxidase is implicated in the pathogenesis of various cardiovascular disorders. In vascular smooth muscle cells (VSMC), expression of NOX1 (NADPH oxidase 1), a catalytic subunit of NADPH oxidase, is low and is induced upon stimulation by vasoactive factors, while it is abundantly expressed in colon epithelial cells. To clarify the regulatory mechanisms underlying such cell-specific expression, the upstream regions directing transcription of the NOX1 gene were explored. In P53LMACO1 cells, a cell line originated from mouse VSMCs, two novel Nox1 mRNA species, the c- and f-type, were isolated. These transcripts contained 5'-untranslated.regions that differed from the colon type mRNA (a-type) and encoded an additional N-terminal peptide of 28 amino acids. When these transcripts were fused to the c-myc tag and expressed in human embryonic kidney 293 cells, a fraction of translated proteins demonstrated the size containing the additional peptide. Proteins encoded by the c- and f-type mRNAs exhibited superoxide-producing activities equivalent to the activity of the a-type form. The a-type mRNA was expressed in the colon and in the intact aorta, whereas the c-type mRNA was detected in the primary cultured VSMCs migrated from aortic explants, in vascular tissue of a wire-injury model and in the thoracic aorta of mice infused with angiotensin II. The promoter region of the c-type mRNA exhibited transcriptional activity in P53LMACO1 cells, but not in MCE301 cells, a mouse colon epithelial cell line. These results suggest that expression of the Nox1 gene is regulated by alternative promoters and that the novel c-type transcript is induced under phenotypic modulation of VSMcs.
引用
收藏
页码:303 / 310
页数:8
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