Use of an on-line imprinted polymer pre-column, for the liquid chromatographic-UV absorbance determination of carbaryl and its metabolite in complex matrices

被引:32
作者
Hantash, Jamil
Bartlett, Alan
Oldfield, Philip
Denes, Georges
O'Rielly, Roger
Roudiere, Daniel
Menduni, Stephanie
机构
[1] Montreal Inc, Charles River Labs, Senneville, PQ H9X 3R3, Canada
[2] Concordia Univ, Lab Solid State Chem & Mossbauer Spect, Dept Chem & Biochem, Montreal, PQ, Canada
关键词
molecular imprinted polymer; liquid chromatography; pesticides; suspension polymerization; detection; HPLC;
D O I
10.1016/j.chroma.2006.05.054
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A carbaryl and 1-naphtol molecular imprinted polymers (MIP1 and MIP2, respectively) were prepared using suspension polymerization and tested for selective and reversible binding to carbaryl and 1-naphtol, respectively. In the suspension polymerization technique used, polymers of methacrylic acid (MAA), highly cross-linked with ethylene dimethacrylate, provide a specific binding sites for the carbaryl molecule and its metabolite when using it as a template to be removed after polymerization. The molecular imprinted polymer with a particle size of similar to 5 mu m were isolated and packed into a pre-column (50 mm x 4.6 mm id) that was used to isolate carbaryl and its metabolite from complex matrices injected on a high performance liquid chromatography system using ultra-violet detection without extensive sample preparation and clean up. The HPLC method had a detection limit of 1.00 ng/mL and a linear response (r(2) > 0.98) over the concentration range of 1.00-10.0 ng/mL. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:104 / 111
页数:8
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