A commentary on "Urea, the most abundant component in urine, cross-reacts with a commercial 8-OH-dG ELISA kit and contributes to overestimation of urinary 8-OH-dG". What is ELISA detecting?

被引:14
作者
Cooke, Marcus S. [1 ,2 ]
机构
[1] Univ Leicester, Dept Canc Studies, Radiat & Oxidat Stress Sect, Kilpatrick Clin Sci Bldg, Leicester LE2 7LX, Leics, England
[2] Univ Leicester, Dept Genet, Leicester LE2 7LX, Leics, England
关键词
PERFORMANCE LIQUID-CHROMATOGRAPHY; OXIDATIVE STRESS; MONOCLONAL-ANTIBODY; DNA; 8-HYDROXY-2'-DEOXYGUANOSINE; DISEASE; MARKERS;
D O I
10.1016/j.freeradbiomed.2009.04.003
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
引用
收藏
页码:30 / 31
页数:2
相关论文
共 28 条
[1]   A carbon column-based liquid chromatography electrochemical approach to routine 8-hydroxy-2 '-deoxyguanosine measurements in urine and other biologic matrices: A one-year evaluation of methods [J].
Bogdanov, MB ;
Beal, MF ;
McCabe, DR ;
Griffin, RM ;
Matson, WR .
FREE RADICAL BIOLOGY AND MEDICINE, 1999, 27 (5-6) :647-666
[2]   Measurement of 8-hydroxy-2′-deoxyguanosine by a commercially available ELISA test:: Comparison with HPLC/electrochemical detection in calf thymus DNA and determination in human serum [J].
Breton, J ;
Sichel, F ;
Bianchini, F ;
Prevost, V .
ANALYTICAL LETTERS, 2003, 36 (01) :123-134
[3]   Urinary 8-hydroxydeoxyguano sine and its analogs as DNA marker of oxidative stress: development of an ELISA and measurement in both bladder and prostate cancers [J].
Chiou, CC ;
Chang, PY ;
Chan, EC ;
Wu, TL ;
Tsao, KC ;
Wu, JT .
CLINICA CHIMICA ACTA, 2003, 334 (1-2) :87-94
[4]   Measurement and meaning of oxidatively modified DNA lesions in urine [J].
Cooke, Marcus S. ;
Olinski, Ryszard ;
Loft, Steffen .
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION, 2008, 17 (01) :3-14
[5]   Interlaboratory comparison of methodologies for the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine [J].
Cooke, Marcus S. ;
Barregard, Lars ;
Mistry, Vilas ;
Potdar, Neelam ;
Rozalski, Rafal ;
Gackowski, Daniel ;
Siomek, Agnieszka ;
Foksinski, Marek ;
Svoboda, Peter ;
Kasai, Hiroshi ;
Konje, Justin C. ;
Sallsten, Gerd ;
Evans, Mark D. ;
Olinski, Ryszard .
BIOMARKERS, 2009, 14 (02) :103-110
[6]   Evidence for attenuated cellular 8-oxo-7,8-dihydro-2′-deoxyguanosine removal in cancer patients [J].
Cooke, MS ;
Rozalski, R ;
Dove, R ;
Gackowski, D ;
Siomek, A ;
Evans, MD ;
Olinski, R .
BIOLOGICAL CHEMISTRY, 2006, 387 (04) :393-400
[7]   Progress in the analysis of urinary oxidative DNA damage [J].
Cooke, MS ;
Lunec, J ;
Evans, MD .
FREE RADICAL BIOLOGY AND MEDICINE, 2002, 33 (12) :1601-1614
[8]   Monoclonal antibody to single-stranded DNA: A potential tool for DNA repair studies [J].
Cooke, MS ;
Patel, K ;
Ahmad, J ;
Holloway, K ;
Evans, MD ;
Lunec, J .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 284 (01) :232-238
[9]   Analysis of urinary 8-oxo-7,8-dihydro-purine-2'-deoxyribonucleosides by LC-MS/MS and improved ELISA [J].
Evans, Mark D. ;
Singh, Rajinder ;
Mistry, Vilas ;
Sandhu, Karendeep ;
Farmer, Peter B. ;
Cooke, Marcus S. .
FREE RADICAL RESEARCH, 2008, 42 (10) :831-840
[10]   Establishing the background level of base oxidation in human lymphocyte DNA: results of an interlaboratory validation study [J].
Gedik, CM ;
Collins, A ;
Dubois, J ;
Duez, P ;
Kouegnigan, L ;
Rees, JF ;
Loft, S ;
Moller, P ;
Jensen, A ;
Poulsen, H ;
Riss, B ;
Weimann, A ;
Cadet, J ;
Douki, T ;
Ravant, JL ;
Sauvaigo, S ;
Faure, H ;
Morel, I ;
Morin, B ;
Epe, B ;
Eckert, I ;
Hartwig, A ;
Schwerdtle, T ;
Dolara, P ;
Giovannelli, L ;
Lodovici, M ;
Guglielmi, F ;
Olinski, R ;
Bialkowski, K ;
Foksinski, M ;
Gackowski, D ;
Duracková, Z ;
Muchová, J ;
Korytar, P ;
Sivonová, M ;
Dusinská, M ;
Mislanová, C ;
Petrovská, H ;
Smolková, B ;
Viña, J ;
Lloret, A ;
Sáez, G ;
Möller, L ;
Hofer, T ;
Eriksson, H ;
Gremaud, E ;
Herbert, K ;
Wild, C ;
Kelly, F ;
Dunster, C .
FASEB JOURNAL, 2005, 19 (01) :82-84