Protective effifect of the Nramp1 BB genotype against Brucella abortus in the water buffalo (Bubalus bubalis)

被引：41

作者：

Capparelli, Rosanna

Alfano, Flora

Amoroso, Maria Grazia

Borriello, Giorgia

Fenizia, Domenico

Bianco, Antonio

Roperto, Sante

Roperto, Franco

Iannelli, Domenico

机构：

[1] Univ Naples Federico II, Cattedra Immunol, Dipartimento Sci Zootecn & Ispez Alimenti, Fac Biotechnol Sci, I-80055 Naples, Italy

[2] Ist Zooprofilatt Sperimentale Mezzogiorno, Portici, Italy

[3] Univ Naples Federico II, Fac Agr, Chair Immunol, I-80055 Naples, Italy

[4] Reg Campania Assessorato Agr, Naples, Italy

[5] Univ Naples Federico II, Fac Vet Med, I-80055 Naples, Italy

来源：

INFECTION AND IMMUNITY | 2007年 / 75卷 / 02期

关键词：

D O I：

10.1128/IAI.00948-06

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti-Brucella abortus antibodies (by the skin test, the agglutination test, and the complement fixation test) and the Nramp1 genotype (by capillary electrophoresis). Four alleles (Nramp1A, -B, -C, and -D) were detected in the 3' untranslated region of the Nramp1 gene. The BB genotype was represented among only controls, providing evidence that this genotype confers resistance to Brucella abortus. The monocytes from the BB (resistant) subjects displayed a higher basal level of Nramp1 mRNA and a lower number of viable intracellular bacteria than did the monocytes from AA (susceptible) subjects. The higher basal level of the antibacterial protein Nramp1 most probably provides the BB animals with the possibility of controlling bacteria immediately after their entry inside the cell.

引用

页码：988 / 996

页数：9