Single-molecule force spectroscopy and imaging of the vancomycin/D-Ala-D-Ala interaction

被引:118
作者
Gilbert, Yann
Deghorain, Marie
Wang, Ling
Xu, Bing
Pollheimer, Philipp D.
Gruber, Hermann J.
Errington, Jeff
Hallet, Bernard
Haulot, Xavier
Verbelen, Claire
Hols, Pascal
Dufrene, Yves F.
机构
[1] Univ Catholique Louvain, Unite Chim & Interfaces, B-1348 Louvain, Belgium
[2] Univ Catholique Louvain, Genet Unit, Inst Sci Vie, B-1348 Louvain, Belgium
[3] Johannes Kepler Univ, Inst Biophys, A-4040 Linz, Austria
[4] Hong Kong Univ Sci & Technol, Dept Chem, Hong Kong, Hong Kong, Peoples R China
[5] Univ Newcastle Upon Tyne, Sch Med, Inst Cell & Mol Biosci, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England
关键词
D O I
10.1021/nl0700853
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
The clinically important vancomycin antibiotic inhibits the growth of pathogens such as Staphylococcus aureus by blocking cell wall synthesis through specific recognition of nascent peptidoglycan terminating in D-Ala-D-Ala. Here, we demonstrate the ability of single-molecule atomic force microscopy with antibiotic-modified tips to measure the specific binding forces of vancomycin and to map individual ligands on living bacteria. The single-molecule approach presented here provides new opportunities for understanding the binding mechanisms of antibiotics and for exploring the architecture of bacterial cell walls.
引用
收藏
页码:796 / 801
页数:6
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