Involvement of the angiotensin II type 2 receptor in apoptosis during human fetal adrenal gland development

被引:41
作者
Chamoux, E
Breault, L
Lehoux, JG
Gallo-Payet, N
机构
[1] Univ Sherbrooke, Fac Med, Dept Med, Serv Endocrinol, Sherbrooke, PQ J1H 5N4, Canada
[2] Univ Sherbrooke, Fac Med, Dept Biochem, Sherbrooke, PQ J1H 5N4, Canada
关键词
D O I
10.1210/jc.84.12.4722
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The aim of this study was to establish a link between the highly expressed angiotensin II (Ang II) type 2 receptor (AT(2)) in human fetal adrenal cells and the proposed apoptotic activity in the center of the gland. There was an important increase in apoptotic DNA fragmentation with age in adrenal glands of fetuses from 15-20 weeks gestation. Adrenal cells showing the characteristic apoptotic internucleosomal DNA fragmentation were localized in the central portion of the fetal zone. In cells cultured for 24 h, Ang II, via the AT(2) receptor, induced DNA fragmentation and cleavage of the DNA repair enzyme, poly-(ADP-ribose) polymerase. Furthermore, characteristic membrane blebbing was observed specifically on cells of the fetal zone. Immunofluorescence studies demonstrated that stimulation with Ang II or CGP 42112 (an agonist of the AT(2) receptor) strongly modified the actin network, now localized exclusively along the plasma membrane, with a predominance of labeling at the base of the bleb formation. This rearrangement of actin distribution was different in cells from the definitive zone, corroborating the observation that these cells express many more Ang II type 1 receptors (AT(1)) than AT(2) receptors. Taken together, our data indicate that the AT(2) receptor is involved in the apoptotic process observed in the human fetal adrenal gland and could participate in the morphological changes occurring after birth, leading to involution of the fetal zone.
引用
收藏
页码:4722 / 4730
页数:9
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共 70 条
  • [1] Angiotensin II type 2 receptors mediate inhibition of mitogen-activated protein kinase cascade and functional activation of SHP-1 tyrosine phosphatase
    Bedecs, K
    Elbaz, N
    Sutren, M
    Masson, M
    Susini, C
    Strosberg, AD
    Nahmias, C
    [J]. BIOCHEMICAL JOURNAL, 1997, 325 : 449 - 454
  • [2] Postnatal involution of the human adrenal fetal zone: Stereologic description and apoptosis
    Bocian-Sobkowska, J
    Wozniak, W
    Malendowicz, LK
    [J]. ENDOCRINE RESEARCH, 1998, 24 (3-4) : 969 - 973
  • [3] The angiotensin AT(2) receptor is present in the human fetal adrenal gland throughout the second trimester of gestation
    Breault, L
    Lehoux, JG
    GalloPayet, N
    [J]. JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1996, 81 (11) : 3914 - 3922
  • [4] Actin is cleaved during constitutive apoptosis
    Brown, SB
    Bailey, K
    Savill, J
    [J]. BIOCHEMICAL JOURNAL, 1997, 323 : 233 - 237
  • [5] Apoptotic cell death in the rat adrenal gland: An in vivo and in vitro investigation
    Carsia, RV
    Macdonald, GJ
    Gibney, JA
    Tilly, KI
    [J]. CELL AND TISSUE RESEARCH, 1996, 283 (02) : 247 - 254
  • [6] Models to elucidate the regulation of adrenal cell death
    Carsia, RV
    Nagele, RG
    Morita, Y
    Tilly, KI
    Tilly, JL
    [J]. ENDOCRINE RESEARCH, 1998, 24 (3-4) : 899 - 908
  • [7] Hormonal modulation of apoptosis in the rat adrenal gland in vitro is dependent on structural integrity
    Carsia, RV
    Tilly, KI
    Tilly, JL
    [J]. ENDOCRINE, 1997, 7 (03) : 377 - 381
  • [8] Comparative effects of ACTH, PACAP, and VIP on fetal human adrenal cells
    Chamoux, E
    Breault, L
    LeHoux, JG
    Gallo-Payet, N
    [J]. ENDOCRINE RESEARCH, 1998, 24 (3-4) : 943 - 946
  • [9] Angiotensin II activates programmed myocyte cell death in vitro
    Cigola, E
    Kajstura, J
    Li, BS
    Meggs, LG
    Anversa, P
    [J]. EXPERIMENTAL CELL RESEARCH, 1997, 231 (02) : 363 - 371
  • [10] ANGIOTENSIN-II STIMULATES GROWTH AND STEROIDOGENESIS IN ZONA-FASCICULATA-RETICULARIS CELLS FROM BOVINE ADRENAL-CORTEX VIA THE AT1 RECEPTOR SUBTYPE
    CLYNE, CD
    NICOL, MR
    MACDONALD, S
    WILLIAMS, BC
    WALKER, SW
    [J]. ENDOCRINOLOGY, 1993, 132 (05) : 2206 - 2212