Meat species identification and Halal authentication analysis using mitochondrial DNA

被引：165

作者：

Murugaiah, Chandrika ^{[2
]}

Noor, Zainon Mohd ^{[3
]}

Mastakim, Maimunah ^{[4
]}

Bilung, Lesley Maurice ^{[5
]}

Selamat, Jinap ^{[1
]}

Radu, Son ^{[1
]}

机构：

[1] Univ Putra Malaysia, Dept Food Sci, Fac Food Sci & Technol, Serdang 43400, Selangor, Malaysia

[2] Univ Sains Malaysia, Sch Hlth Sci, Kubang Kerian 16150, Kelantan, Malaysia

[3] Univ Teknol MARA, Fac Sci Appl, Shah Alam 40450, Selangor, Malaysia

[4] Univ Teknol MARA, Fac Hlth Sci, Petaling Jaya 46000, Selangor, Malaysia

[5] Univ Malaysia Sarawak, Dept Mol Biol, Fac Sci & Resource Technol, Kota Samarahan 94300, Sarawak, Malaysia

来源：

MEAT SCIENCE | 2009年 / 83卷 / 01期

关键词：

Cyt b gene; Mitochondrial DNA; Halal authentication; Forensic science; Meat; PCR-restriction fragment length polymorphism (RFLP); Species identification; POLYMERASE-CHAIN-REACTION; LENGTH-POLYMORPHISM ANALYSIS; RIBOSOMAL-RNA GENE; MOLECULAR-IDENTIFICATION; PCR AMPLIFICATION; ANIMAL ORIGIN; PRODUCTS; PORK; EVOLUTION; BEEF;

D O I：

10.1016/j.meatsci.2009.03.015

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A method utilizing PCR-restriction fragment length polymorphism (RFLP) in the mitochondrial genes was developed for beef (Bos taurus), pork (Sus scrofa), buffalo (Bubalus bubali), quail (Coturnix coturnix), chicken (Gallus gallus), goat (Capra hircus), rabbit (Oryctolagus cuniculus) species identification and Halal authentication. PCR products of 359-bp were successfully obtained from the cyt b gene of these six meats. Alu1, BsofI, Rsa1, Mse1, and BstU1 enzymes were identified as potential restriction endonucleases to differentiate the meats. The genetic differences within the cyt b gene among the meat were successfully confirmed by PCR-RFLP. A reliable typing scheme of species which revealed the genetic differences among the species was developed. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：57 / 61

页数：5

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