Detection of all seven serotypes of foot-and-mouth disease virus by real-time, fluorogenic reverse transcription polymerase chain reaction assay

被引：162

作者：

Reid, SM ^{[1
]}

Ferris, NP ^{[1
]}

Hutchings, GH ^{[1
]}

Zhang, ZD ^{[1
]}

Belsham, GJ ^{[1
]}

Alexandersen, S ^{[1
]}

机构：

[1] AFRC, Inst Anim Hlth, Pirbright Lab, Woking GU24 0NF, Surrey, England

来源：

JOURNAL OF VIROLOGICAL METHODS | 2002年 / 105卷 / 01期

关键词：

foot-and-mouth disease virus; diagnosis; reverse transcription polymerase chain reaction; fluorogenic RT-PCR assay; quantitative RT-PCR;

D O I：

10.1016/S0166-0934(02)00081-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A fluorogenic RT-PCR (5'-nuclease probe-based) assay using a primer/probe set designed from the internal ribosomal entry site region of the virus genome was developed for the specific detection of all seven serotypes of foot-and-mouth disease (FMD) virus in epithelial suspensions and cell culture virus preparations. The reverse transcription polymerase chain reaction (RT-PCR) specifically detected FMD virus in sample submissions from the UK 2001 FMD outbreak with greater sensitivity than our conventional RT-PCR procedure and our routine diagnostic procedures of ELISA and virus isolation in cell culture. The fluorogenic RT-PCR provides relatively fast results, enables a quantitative assessment to be made of virus amounts and can handle more samples and/or replicates of samples in a single assay than the conventional RT-PCR procedure, Therefore it is seen as a valuable tool to complement the routine diagnostic procedures for FMD virus diagnosis. (C) 2002 Published by Elsevier Science B.V.

引用

页码：67 / 80

页数：14