RNA-selective, live cell imaging probes for studying nuclear structure and function

被引:172
作者
Li, Qian
Kim, Yunkyung
Namm, Joshua
Kulkarni, Amita
Rosania, Gus R.
Ahn, Young-Hoon
Chang, Young-Tae
机构
[1] NYU, Dept Chem, New York, NY 10003 USA
[2] Univ Michigan, Coll Pharm, Dept Pharmaceut Sci, Ann Arbor, MI 48109 USA
来源
CHEMISTRY & BIOLOGY | 2006年 / 13卷 / 06期
关键词
D O I
10.1016/j.chembiol.2006.04.007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The higher-order structural organization of the cell nucleus reflects the underlying genome-wide transcriptional activity and macromolecular transport processes. To study the microscopic organization of RNA distribution within the nucleus, a combinatorial library of fluorescent styryl molecules was synthesized and screened for an in vitro RNA response and live cell nuclear imaging. Four different cell lines (HeLa, A549, 3T3, and 3T3-L1) were analyzed in terms of higher-order nuclear organization. We identified RNA-selective dyes with better imaging properties relative to commercially available SYTORNASelect dye; the selected dyes were also cell permeant, photostable, and well tolerated by the cells. Our dyes also had very good counterstain compatibility with Hoechst and DAPI, which could help to image the DNA distribution in relation to RNA distribution in live cells and therefore reveal different patterns of RNA-DNA colocalization.
引用
收藏
页码:615 / 623
页数:9
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