Cloning and sequencing of the cellular-viral junctions from the human adenovirus type 5 transformed 293 cell line

被引：158

作者：

Louis, N

Evelegh, C

Graham, FL

机构：

[1] MCMASTER UNIV,DEPT BIOL,HAMILTON,ON L8S 4K1,CANADA

[2] MCMASTER UNIV,DEPT PATHOL,HAMILTON,ON L8S 4K1,CANADA

来源：

VIROLOGY | 1997年 / 233卷 / 02期

基金：

加拿大自然科学与工程研究理事会; 英国医学研究理事会; 美国国家卫生研究院;

关键词：

D O I：

10.1006/viro.1997.8597

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The human-viral junctions of integrated adenovirus type 5 (Ad5) DNA in 293 cells have been cloned and sequenced. The Ad5 sequences extend from nucleotides (nt) 1 to 4344 and are located in the pregnancy-specific beta-1-glycoprotein 4 (PSG 4) gene. This maps the insertion of Ad5 DNA to human chromosome 19 (19q13.2). The Ad5 sequences are represented as a single collinear insertion of viral DNA with no rearrangements. There were 19 bp of PSG4 DNA deleted at the site of insertion and an extra 3 nt (GTC) at the left viral/cellular junction. A short patch of homology between viral DNA and PSG4 DNA (6 nt with 1 mismatch) was present at the right junction. (C) 1997 Academic Press.

引用

页码：423 / 429

页数：7

共 26 条

[1] ADENOVIRUS 5 DNA SEQUENCES PRESENT AND RNA SEQUENCES TRANSCRIBED IN TRANSFORMED HUMAN-EMBRYO KIDNEY-CELLS (HEK-AD-5 OR 293) [J].