Nuclear reassortment between vegetative mycelia in natural populations of the basidiomycete Heterobasidion annosum

被引:48
作者
Johannesson, H
Stenlid, J
机构
[1] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA
[2] Swedish Univ Agr Sci, Dept Forest Mycol & Pathol, S-75007 Uppsala, Sweden
关键词
nuclear mosaic; nuclear reassortment; somatic incompatibility; microsatellites; root rot; natural chimera; fungi; allorecognition; individual; operational selective unit;
D O I
10.1016/j.fgb.2004.01.002
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Somatic incompatibility is not an absolute block to nuclear exchange between incompatible mycelia of the basidiomycete Heterobasidion annosum in vitro. Under laboratory conditions new heterokaryotic genotypes can be isolated from the gap between incompatible heterokaryons, and nuclear migration between pairs of heterokaryons grown in Petri dishes has been observed. In this study, we test the hypothesis of nuclear transfer and reassortment between heterokaryotic mycelia in natural populations of H. annosum. We developed six microsatellite markers to genotype nuclei populating 21 somatically incompatible mycelia of H. annosum isolated from a single stump of Picea abies, and found that the detected heterokaryons share nuclei; 10 of the nuclear haplotypes were found in more than one mycelium. In one isolate, four nuclear types were found in the same mycelium. These findings indicate that new heterokaryons can be formed as a result of nuclear reassortment between incompatible heterokaryotic mycelia in nature. (C) 2004 Elsevier Inc. All rights reserved.
引用
收藏
页码:563 / 570
页数:8
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