Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins

被引：62

作者：

Lyman, SK

Gerace, L

Baserga, SJ

机构：

[1] Yale Univ, Sch Med, Dept Therapeut Radiol, New Haven, CT 06520 USA

[2] Yale Univ, Sch Med, Dept Genet, New Haven, CT 06520 USA

[3] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

[4] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

来源：

RNA | 1999年 / 5卷 / 12期

关键词：

genomics; nucleolus; ribosome biogenesis; snoRNPs;

D O I：

10.1017/S1355838299991288

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified an apparent human homolog of the yeast Nop5/Nop58 protein. hNop5/Nop58 codes for a protein of predicted molecular weight 59.6 kDa and is 46.8% identical to Saccharomyces cerevisiae Nop5/Nop58. Immunofluorescent staining with antibodies against hNop5/Nop58 indicate that it is localized primarily to the nucleolus, and coimmunoprecipitation from nuclear extracts demonstrates that hNop5/Nop58 interacts with the box C/D family of snoRNAs. Thus, hNop5/Nop58 is a common component of the box C/D snoRNPs, and joins fibrillarin as the second such component identified and characterized in metazoans.

引用

页码：1597 / 1604

页数：8

共 33 条

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