Cloning and characterization of the first two genes of the non-oxidative part of the Saccharomyces cerevisiae pentose-phosphate pathway

被引：30

作者：

Miosga, T ^{[1
]}

Zimmermann, FK ^{[1
]}

机构：

[1] TECH UNIV DARMSTADT,INST MIKROBIOL & GENET,D-64287 DARMSTADT,GERMANY

来源：

CURRENT GENETICS | 1996年 / 30卷 / 05期

关键词：

D-ribulose-5-phosphate; 3-epimerase; D-ribose-5-phosphate ketol-isomerase; pentose-phosphate pathway; Saccharomyces cerevisiae;

D O I：

10.1007/s002940050149

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have cloned and characterized the two remaining unknown genes of the non-oxidative part of the pentose-phosphate pathway of Saccharomyces cerevisiae encoding the enzymes D-ribulose-5-phosphate 3-epimerase (Rpe1p) and D-ribose-5-phosphate ketol-isomerase (Rki1p). Rpe1p has an unexpected high specific activity of 2148 mUx(mg protein)(-1) in crude extracts. Deletion mutants of RPE1 show no enzyme activity and are unable to grow on D-xylulose. Unexpectedly haploid rki1 deletion mutants are not viable. Functional expression of RKI1 was demonstrated following an increase of gene dosage in the haploid rki1 deletion mutant, which restored viability and specific D-ribose-5-phosphate ketol-isomerase activity. Both enzymes show high similarity to the deduced protein sequences of various open reading frames, expressed sequence tags or cDNAs from different organisms.

引用

页码：404 / 409

页数：6

共 50 条

[1] THE RIBOSE 5-PHOSPHATE ISOMERASE-ENCODING GENE IS LOCATED IMMEDIATELY DOWNSTREAM FROM THAT ENCODING MURINE IMMUNOGLOBULIN-KAPPA [J].

APEL, TW ;

SCHERER, A ;

ADACHI, T ;

AUCH, D ;

AYANE, M ;

RETH, M .

GENE, 1995, 156 (02) :191-197

[2]

BENNETZEN JL, 1982, J BIOL CHEM, V257, P3026

[3] OVALBUMIN GENE-SEQUENCE OF PUTATIVE CONTROL REGIONS [J].

BENOIST, C ;

OHARE, K ;

BREATHNACH, R ;

CHAMBON, P .

NUCLEIC ACIDS RESEARCH, 1980, 8 (01) :127-142

[4]

BLATTNER FR, 1993, NUCLEIC ACIDS RES, V21, P5408

[5] A FAMILY OF HEXOSEPHOSPHATE MUTASES IN SACCHAROMYCES-CEREVISIAE [J].

BOLES, E ;

LIEBETRAU, W ;

HOFMANN, M ;

ZIMMERMANN, FK .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 220 (01) :83-96

[6] THE ROLE OF THE NAD-DEPENDENT GLUTAMATE-DEHYDROGENASE IN RESTORING GROWTH ON GLUCOSE OF A SACCHAROMYCES-CEREVISIAE PHOSPHOGLUCOSE ISOMERASE MUTANT [J].

BOLES, E ;

LEHNERT, W ;

ZIMMERMANN, FK .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 217 (01) :469-477

[7] STERILE HOST YEASTS (SHY) - EUKARYOTIC SYSTEM OF BIOLOGICAL CONTAINMENT FOR RECOMBINANT DNA EXPERIMENTS [J].

BOTSTEIN, D ;

FALCO, SC ;

STEWART, SE ;

BRENNAN, M ;

SCHERER, S ;

STINCHCOMB, DT ;

STRUHL, K ;

DAVIS, RW .

GENE, 1979, 8 (01) :17-24

[8] ANALYSIS OF THE ESCHERICHIA-COLI GENOME .6. DNA-SEQUENCE OF THE REGION FROM 92.8 THROUGH 100 MINUTES [J].

BURLAND, V ;

PLUNKETT, G ;

SOFIA, HJ ;

DANIELS, DL ;

BLATTNER, FR .

NUCLEIC ACIDS RESEARCH, 1995, 23 (12) :2105-2119

[9] PHYSIOLOGICAL-EFFECTS OF 7 DIFFERENT BLOCKS IN GLYCOLYSIS IN SACCHAROMYCES-CEREVISIAE [J].

CIRIACY, M ;

BREITENBACH, I .

JOURNAL OF BACTERIOLOGY, 1979, 139 (01) :152-160

[10] CONSERVATION OF HIGH-EFFICIENCY PROMOTER SEQUENCES IN SACCHAROMYCES-CEREVISIAE [J].

DOBSON, MJ ;

TUITE, MF ;

ROBERTS, NA ;

KINGSMAN, AJ ;

KINGSMAN, SM ;

PERKINS, RE ;

CONROY, SC ;

DUNBAR, B ;

FOTHERGILL, LA .

NUCLEIC ACIDS RESEARCH, 1982, 10 (08) :2625-2637

← 1 2 3 4 5 →