Characterization of a novel cry8 gene specific to Melolonthidae pests: Holotrichia oblita and Holotrichia parallela

被引：45

作者：

Shu, Changlong ^{[1
]}

Yan, Guixin ^{[1
]}

Wang, Rongyan ^{[2
]}

Zhang, Jie ^{[1
]}

Feng, Shuliang ^{[2
]}

Huang, Dafang ^{[3
]}

Song, Fuping ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

[2] Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Beijing 071000, Peoples R China

[3] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2009年 / 84卷 / 04期

关键词：

PCR-RFLP; Bacillus thuringiensis; Cry8Ga; Holotrichia oblita; Holotrichia parallela; BACILLUS-THURINGIENSIS STRAIN; INSECTICIDAL ACTIVITY; ANOMALA-CORPULENTA; IDENTIFICATION; SCARABAEIDAE; TOXICITY; PROTEINS; INSECTS; CLONING; LARVAE;

D O I：

10.1007/s00253-009-1971-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new polymerase chain reaction-restriction fragment length polymorphism method for the identification of cry8-type genes from Bacillus thuringiensis has been established by designing a pair of new universal primers. By this method, a novel gene, cry8Ga1, encoding a polypeptide of 1,157 amino acids with a deduced molecular mass of 131.2 kDa was identified and cloned from B. thuringiensis HBF-18. Recombinant B. thuringiensis strain HD8G, harboring cry8Ga1, has insecticidal activity against larvae of Melolonthidae pests: Holotrichia oblita and Holotrichia parallela. This is the first report of a Cry toxin that has insecticidal activity to Melolonthidae pest H. oblita.

引用

页码：701 / 707

页数：7

共 21 条

[1] A strain of Bacillus thuringiensis subsp galleriae containing a novel cry8 gene highly toxic to Anomala cuprea (Coleoptera: Scarabaeidae) [J].