Wnt2/2b and β-Catenin Signaling Are Necessary and Sufficient to Specify Lung Progenitors in the Foregut

被引：337

作者：

Goss, Ashley M. ^{[1
,2
]}

Tian, Ying ^{[1
,3
]}

Tsukiyama, Tadasuke ^{[4
]}

Cohen, Ethan David ^{[1
,3
]}

Zhou, Diane ^{[1
,3
]}

Lu, Min Min ^{[1
,3
]}

Yamaguchi, Terry P. ^{[4
]}

Morrisey, Edward E. ^{[1
,2
,3
,5
]}

机构：

[1] Univ Penn, Dept Med, Philadelphia, PA 19104 USA

[2] Univ Penn, Dept Cell & Dev Biol, Philadelphia, PA 19104 USA

[3] Univ Penn, Cardiovasc Inst, Philadelphia, PA 19104 USA

[4] NCI, Canc & Dev Biol Lab, NIH, Frederick, MD 21702 USA

[5] Univ Penn, Inst Regenerat Med, Philadelphia, PA 19104 USA

来源：

DEVELOPMENTAL CELL | 2009年 / 17卷 / 02期

基金：

美国国家卫生研究院;

关键词：

ENHANCER-BINDING PROTEIN; LIVER GROWTH; WNT SIGNALS; MORPHOGENESIS; GENE; EPITHELIUM; PROLIFERATION; SPECIFICATION; DELETION; CELLS;

D O I：

10.1016/j.devcel.2009.06.005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Patterning of the primitive foregut promotes appropriate organ specification along its anterior-posterior axis. However, the molecular pathways specifying foregut endoderm progenitors are poorly understood. We show here that Wnt2/2b signaling is required to specify lung endoderm progenitors within the anterior foregut. Embryos lacking Wnt2/2b expression exhibit complete lung agenesis and do not express Nkx2.1, the earliest marker of the lung endoderm. In contrast, other foregut endoderm-derived organs, including the thyroid, liver, and pancreas, are correctly specified. The phenotype observed is recapitulated by an endoderm-restricted deletion of beta-catenin, demonstrating that Wnt2/2b signaling through the canonical Writ pathway is required to specify lung endoderm progenitors within the foregut. Moreover, activation of canonical Wnt/beta-catenin signaling results in the reprogramming of esophagus and stomach endoderm to a lung endoderm progenitor fate. Together, these data reveal that canonical Wnt2/2b signaling is required for the specification of lung endoderm progenitors in the developing foregut.

引用

页码：290 / 298

页数：9

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