Evaluation of cell numbers and viability of Saccharomyces cerevisiae by different counting methods

被引：20

作者：

Fiala, J

Lloyd, DR

Rychtera, M

Kent, CA ^{[1
]}

Al-Rubeai, M

机构：

[1] Univ Birmingham, Sch Chem Engn, Ctr Bioproc Engn, Birmingham B15 2TT, W Midlands, England

[2] Inst Chem Technol, Dept Fermentat Chem & Bioengn, Prague 16628 6, Czech Republic

来源：

BIOTECHNOLOGY TECHNIQUES | 1999年 / 13卷 / 11期

基金：

英国生物技术与生命科学研究理事会;

关键词：

cell number; Coulter principle; flow cytometry; viability; yeast;

D O I：

10.1023/A:1008974419342

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Four counting methods (two flow cytometry, one Coulter principle, one microscopic) are compared for measuring cell density and viability of batch-grown yeast. All gave adequate precision in measuring total cell density with no systematic difference between methods. However, the promise of flow cytometry as a rapid means of determining both quantity and quality of a cell population is shown.

引用

页码：787 / 795

页数：9

共 12 条

[1] A rapid method for evaluation of cell number and viability by flow cytometry [J].