Rapid susceptibility testing of fungi by flow cytometry using vital staining

被引：49

作者：

Wenisch, C

Linnau, KF

Parschalk, B

ZedtwitzLiebenstein, K

Georgopoulos, A

机构：

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 01期

关键词：

D O I：

10.1128/JCM.35.1.5-10.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 1-h assay for antifungal susceptibility testing measuring the impairment of fungal metabolic activity was developed. Yeast viability was analyzed by flow cytometry with a novel fluorescent probe, FUN-1, which emits a red fluorescence when the yeast is metabolically active. For nine Candida albicans strains tested, this method yielded results comparable to those obtained by the standard M27 procedure for amphotericin B, flucytosine, fluconazole, and ketoconazole, Whether the flow cytometry antifungal susceptibility test results correlate with the in vivo activities of the drugs remains to determined.

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页码：5 / 10

页数：6

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